Saberi Samaneh, Schmidt Alexej, Eybpoosh Sana, Esmaili Maryam, Talebkhan Yeganeh, Mohajerani Nazanin, Oghalaie Akbar, Eshagh Hosseini Mahmoud, Mohagheghi Mohammad Ali, Bugaytova Jeanna, Borén Thomas, Mohammadi Marjan
HPGC Group, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, 1316943551, Iran.
Department of Medical Biosciences and Pathology, Umeå University, 901 85, Umeå, Sweden.
Dig Dis Sci. 2016 Oct;61(10):2868-2877. doi: 10.1007/s10620-016-4217-z. Epub 2016 Jun 18.
BabA is a Helicobacter pylori cell surface adhesin, which binds to the ABO/Le(b) histo-blood group antigens (Le(b)) and serves as a virulence factor.
H. pylori single colonies were isolated from 156 [non-ulcer dyspepsia (NUD) = 97, duodenal ulcer (DU) = 34, gastric cancer (GC) = 25)] patients. babA and babB genes were evaluated by gene/locus-specific PCR. BabA protein expression and Le(b) binding activity were determined by immunoblotting and ELISA, respectively.
The combined categorization of H. pylori strains based on high, low or no levels of BabA expression and Le(b) binding, produced 4 groups: (I) BabA-high/Le(b)-high (36 %), (II) BabA-low/Le(b)-low (26 %), (III) BabA-neg/Le(b)-low (30 %) and (IV) BabA-neg/Le(b)-neg (8 %) strains. The majority (63 %) of the BabA-low/Le(b)-low strains exhibited mixed babA/B genotypes as compared to merely 18 % of the BabA-high/Le(b)-high, 15 % of the BabA-neg/Le(b)-neg and 11 % of the BabA-neg/Le(b)-low (P = 0.0001) strains. In contrast to NUD strains, the great majority (70 %) of DU strains were BabA-low/Le(b)-low (11 %, P = 0.0001), which compared to NUD strains, enhanced the risk of DU by 18.8-fold. In parallel, infection with babA/B mixed genotype strains amplified the risk of DU by 3.6-fold (vs. babA-positive: P = 0.01) to 6.9-fold (vs. babA-negative: P = 0.007).
Here, we show higher prevalence of mixed babA/B genotypes among BabA-low/Le(b)-low clinical strains. Recombination of babA and babB genes across their loci may yield lower BabA expression and lower Le(b) binding activity. We conclude that H. pylori strains with lower Le(b) binding activity are better adapted for colonization of the gastric metaplastic patches in the duodenum and enhance the risk of duodenal ulcers.
BabA是幽门螺杆菌细胞表面黏附素，可与ABO/Le(b)组织血型抗原（Le(b)）结合，是一种毒力因子。
从156例患者[非溃疡性消化不良（NUD）=97例，十二指肠溃疡（DU）=34例，胃癌（GC）=25例]中分离出幽门螺杆菌单菌落。通过基因/位点特异性PCR评估babA和babB基因。分别通过免疫印迹和ELISA测定BabA蛋白表达和Le(b)结合活性。
根据BabA表达水平高、低或无以及Le(b)结合情况对幽门螺杆菌菌株进行综合分类，产生4组：（I）BabA高/Le(b)高（36%），（II）BabA低/Le(b)低（26%），（III）BabA阴性/Le(b)低（30%）和（IV）BabA阴性/Le(b)阴性（8%）菌株。与BabA高/Le(b)高菌株的18%、BabA阴性/Le(b)阴性菌株的15%和BabA阴性/Le(b)低菌株的11%相比，大多数（63%）BabA低/Le(b)低菌株表现出babA/B混合基因型（P = 0.0001）。与NUD菌株相比，绝大多数（70%）DU菌株为BabA低/Le(b)低（11%，P = 0.0001），与NUD菌株相比，DU风险增加了18.8倍。同时，感染babA/B混合基因型菌株使DU风险增加3.6倍（与babA阳性相比：P = 0.01）至6.9倍（与babA阴性相比：P = 0.007）。
在此，我们显示在BabA低/Le(b)低临床菌株中，babA/B混合基因型的患病率较高。babA和babB基因跨位点重组可能导致BabA表达降低和Le(b)结合活性降低。我们得出结论，Le(b)结合活性较低的幽门螺杆菌菌株更适合在十二指肠胃化生斑块中定植，并增加十二指肠溃疡的风险。
