Cai Xia, Wang Shui-Cai, He Jun-Fang, Liu Xiao, Peng Ju-Fang, Kuang Ting-Yun
College of Life Sciences, Northwest University, Xi'an 710069, China.
Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao. 2006 Apr;32(2):169-74.
Spectroscopic properties within the core complex of photosystem II were investigated by studying the influence of the wavelength of excitation on the fluorescence emission spectrum. At two temperatures, when the core complex of PSII isolated from spinach was excited at six different excitation wavelengths ranging from 436 nm to 520 nm, there is no difference in the maxima of the emission spectra of the core complex, and when the core complex was excited at 480, 489, 495 and 507 nm respectively, fluorescence intensities of maxima decrease with increasing of the absorbance of the beta-carotene molecules at the four excitation wavelengths. The extent of change of the shoulder of the spectra beyond 700 nm depends on the kind of pigment molecule excited. The excitation wavelength can influence the way of energy transfer in the core complex of photosystem II. By Gaussian deconvolution analysis, at least seven groups of chlorophyll a molecules were discovered. They are Chl a(660), Chl a(670), Chl a(680), Chl a(682), Chl a(684), Chl a(687) and Chl a(690).
通过研究激发波长对荧光发射光谱的影响，对光系统II核心复合物的光谱性质进行了研究。在两个温度下，当从菠菜中分离出的光系统II核心复合物在436纳米至520纳米的六个不同激发波长下激发时，核心复合物发射光谱的最大值没有差异，并且当核心复合物分别在480、489、495和507纳米激发时，在这四个激发波长下，最大值处的荧光强度随着β-胡萝卜素分子吸光度的增加而降低。700纳米以上光谱肩峰的变化程度取决于被激发的色素分子种类。激发波长可以影响光系统II核心复合物中的能量转移方式。通过高斯去卷积分析，至少发现了七组叶绿素a分子。它们是叶绿素a(660)、叶绿素a(670)、叶绿素a(680)、叶绿素a(682)、叶绿素a(684)、叶绿素a(687)和叶绿素a(690)。
