Parildar-Karpuzoğlu H, Doğru-Abbasoğlu S, Balkan J, Aykaç-Toker G, Uysal M
Department of Biochemistry, Istanbul Faculty of Medicine, Istanbul University, Capa, Istanbul, Turkey.
Amino Acids. 2007 Jan;32(1):115-9. doi: 10.1007/s00726-005-0282-x. Epub 2006 Apr 20.
We aimed to investigate the effect of decreased taurine levels on endogenous and induced lipid peroxide levels in liver, brain, heart and erythrocytes as well as prooxidant and antioxidant balance in the liver of rats administered beta-alanine (3%, w/v) in drinking water for 1 month to decrease taurine levels of tissues. This treatment caused significant decreases in taurine levels of liver (86%), brain (36%) and heart (15%). We found that endogenous and ascorbic acid-, NADPH- and cumene hydroperoxide-induced malondialdehyde (MDA) levels did not change in the liver, brain and heart homogenates following beta-alanine treatment. Also, H(2)O(2)-induced MDA levels remained unchanged in erythrocytes. In addition, we did not observe any changes in levels of MDA, diene conjugates, glutathione, alpha-tocopherol, ascorbic acid and the activities of superoxide dismutase, glutathione peroxidase and glutathione transferase in the liver. According to this, buffering or sequestering capacity of tissues to exogenous stimuli was not influenced by reduced taurine levels in tissues of rats.
我们旨在研究牛磺酸水平降低对肝脏、大脑、心脏和红细胞中内源性和诱导性脂质过氧化物水平的影响，以及对饮用含β-丙氨酸（3%，w/v）的水1个月以降低组织牛磺酸水平的大鼠肝脏中促氧化剂和抗氧化剂平衡的影响。这种处理导致肝脏（86%）、大脑（36%）和心脏（15%）的牛磺酸水平显著降低。我们发现，在β-丙氨酸处理后，肝脏、大脑和心脏匀浆中内源性以及抗坏血酸、NADPH和氢过氧化异丙苯诱导的丙二醛（MDA）水平没有变化。此外，H₂O₂诱导的红细胞中MDA水平也保持不变。另外，我们未观察到肝脏中MDA、二烯共轭物、谷胱甘肽、α-生育酚、抗坏血酸水平以及超氧化物歧化酶、谷胱甘肽过氧化物酶和谷胱甘肽转移酶活性有任何变化。据此，大鼠组织中牛磺酸水平降低并未影响组织对外源刺激的缓冲或螯合能力。
