Parildar-Karpuzoğlu Hande, Mehmetçik Güldal, Ozdemirler-Erata Gül, Doğru-Abbasoğlu Semra, Koçak-Toker Necla, Uysal Müjdat
Department of Biochemistry, Istanbul Medical Faculty, Istanbul University, Capa, 34093, Istanbul, Turkey.
Pharmacol Rep. 2008 Sep-Oct;60(5):673-8.
The effect of taurine treatment on antioxidant defense in liver and brain tissues of old rats was investigated. Endogenous malondialdehyde (MDA) and diene conjugate (DC), ascorbic acid (AA)- and NADPH-induced lipid peroxide levels as well as non-enzymatic (glutathione--GSH, vitamin E and vitamin C) and enzymatic antioxidants (superoxide dismutase, glutathione peroxidase and glutathione transferase) were determined in livers and brains of young (5 months), old (22 months), and taurine-treated old rats. Taurine (2%, w/v; in drinking water) was administered to old rats for 6 weeks. Taurine levels decreased in the liver and brain of old rats compared to young rats.MDA and DC levels increased, GSH levels decreased, but induced lipid peroxidation remained unchanged in livers of aged rats. Oxidative stress parameters did not change in brains of aged rats. Taurine treatment resulted in significant increases in taurine levels, decreases in MDA and DC levels and increases in GSH levels in livers of old rats. Taurine treatment also increased brain taurine levels. However, no significant changes were detected in lipid peroxidation and antioxidant system in brains of old rats following taurine treatment. Accordingly, in old rats the liver seems more susceptible to age-related lipid peroxidation increases and taurine level changes than the brain. Thus, taurine supplementation seems to be useful for decreasing hepatic oxidative stress in aging.
研究了牛磺酸处理对老年大鼠肝脏和脑组织抗氧化防御的影响。测定了年轻（5个月）、老年（22个月）和牛磺酸处理的老年大鼠肝脏和脑组织中的内源性丙二醛（MDA）和二烯共轭物（DC）、抗坏血酸（AA）和NADPH诱导的脂质过氧化物水平以及非酶抗氧化剂（谷胱甘肽-GSH、维生素E和维生素C）和酶抗氧化剂（超氧化物歧化酶、谷胱甘肽过氧化物酶和谷胱甘肽转移酶）。将牛磺酸（2%，w/v；溶于饮用水）给予老年大鼠6周。与年轻大鼠相比，老年大鼠肝脏和脑组织中的牛磺酸水平降低。老年大鼠肝脏中MDA和DC水平升高，GSH水平降低，但诱导的脂质过氧化保持不变。老年大鼠脑组织中的氧化应激参数没有变化。牛磺酸处理导致老年大鼠肝脏中牛磺酸水平显著升高，MDA和DC水平降低，GSH水平升高。牛磺酸处理还提高了脑牛磺酸水平。然而，牛磺酸处理后老年大鼠脑组织中的脂质过氧化和抗氧化系统未检测到显著变化。因此，在老年大鼠中，肝脏似乎比大脑更容易受到与年龄相关的脂质过氧化增加和牛磺酸水平变化的影响。因此，补充牛磺酸似乎有助于降低衰老过程中的肝脏氧化应激。
