Elhottová Dana, Kristůfek Václav, Frouz Jan, Nováková Alena, Chronáková Alica
Institute of Soil Biology, Academy of Sciences of the Czech Republic, Na Sádkách 7, 370 05, Ceské Budejovice, Czech Republic.
Antonie Van Leeuwenhoek. 2006 Apr-May;89(3-4):459-63. doi: 10.1007/s10482-005-9044-8. Epub 2006 Apr 19.
Viable microorganisms were found in Miocene lacustrine clays of the cypris formation excavated from 200-m below the surface as spoil during open-cast brown coal mining (Sokolov Brown Coal Basin, North-Western Bohemia, Czech Republic). Both saprotrophic microfungi of the genera Penicillium, Verticillium, Cladosporium and Aspergillus as well as heterotrophic bacteria were isolated from an intact sediment cores. Heterotrophic bacteria were classified by the MIS Sherlock System as representatives of genera Nocardiopsis, Arthrobacter, Micrococcus, Kocuria, Rothia, Clavibacter, Bacillus, Paenibacillus, Brevibacillus, Microbacterium, Acinetobacter and Pseudomonas. A bacterium found among the strains had an atypical fatty acids profile enriched by branched fatty acids and polyunsaturated fatty acid (18:3 omega 6) and gave no MIS Sherlock match. Phospholipid fatty acids analysis indicates a relatively high (535 pmol g(-1)) but inhomogeneously distributed viable microbial biomass. Fatty acids analyses of non-fractioned lipids (representing viable, storage and dead biomass; 8390 pmol g(-1)) detected rich and homogenous profiles with fungal, bacterial and actinomycetal markers but no protozoan and algal fatty acids markers.
在露天褐煤开采（捷克共和国西北波希米亚的索科洛夫褐煤盆地）过程中作为废土从地表以下200米处挖掘出的赛氏组中新世湖相粘土中发现了活的微生物。从完整的沉积物岩心中分离出了青霉属、轮枝孢属、枝孢属和曲霉属的腐生微真菌以及异养细菌。异养细菌通过MIS Sherlock系统分类为诺卡氏菌属、节杆菌属、微球菌属、考克氏菌属、罗氏菌属、棒状杆菌属、芽孢杆菌属、类芽孢杆菌属、短短芽孢杆菌属、微杆菌属、不动杆菌属和假单胞菌属的代表。在这些菌株中发现的一种细菌具有非典型的脂肪酸谱，富含支链脂肪酸和多不饱和脂肪酸（18:3 ω6），并且与MIS Sherlock系统不匹配。磷脂脂肪酸分析表明存在相对较高（535 pmol g(-1)）但分布不均匀的活微生物生物量。对未分离的脂质（代表活的、储存的和死亡的生物量；8390 pmol g(-1)）进行的脂肪酸分析检测到了富含真菌、细菌和放线菌标记物的丰富且均匀的图谱，但没有原生动物和藻类脂肪酸标记物。
