Pokorný Richard, Olejníková Petra, Balog Miroslav, Zifcák Peter, Hölker Udo, Janssen Martina, Bend Jutta, Höfer Milan, Holiencin Rudolf, Hudecová Daniela, Varecka L'udovít
Department of Biochemistry and Microbiology, Slovak University of Technology, Radlinského 9, 81237 Bratislava, Slovakia.
Res Microbiol. 2005 Nov;156(9):932-43. doi: 10.1016/j.resmic.2005.04.010. Epub 2005 Jul 21.
Microorganisms were isolated from lignite freshly excavated in the Záhorie coal mine (southwestern Slovakia) under conditions excluding contamination with either soil or air-borne microorganisms. The isolates represented both Prokarya and Eukarya (fungi). All were able to grow on standard media, although some microorganisms were unstable and became extinct during storage of coal samples. Bacteria belonged to the genera Bacillus, Staphylococcus, and Rhodococcus, according to both morphological criteria and ITS sequences. Several bacterial isolates were resistant to antibiotics. The presence of anaerobic bacteria was also documented, although they have not yet been identified. Fungal isolates were typified by using their ITS sequences. They belonged to the genera Trichoderma (Hypocrea), Penicillium, Epicoccum, Metarhizium (Cordyceps), and Cladosporium. Several fungi produced compounds with antibiotic action against standard bacterial strains. The evidence for the presence of microorganisms in native lignite was obtained by means of fluorescence microscopy, scanning electron microscopy, and electron microprobe analysis. Results demonstrated that microorganisms were able to survive in the low-rank coal over a long time period.
在排除土壤或空气传播微生物污染的条件下，从斯洛伐克西南部扎霍里煤矿新开采的褐煤中分离出微生物。分离出的微生物包括原核生物和真核生物（真菌）。所有分离出的微生物都能在标准培养基上生长，不过有些微生物不稳定，在煤样储存期间灭绝了。根据形态学标准和ITS序列，细菌属于芽孢杆菌属、葡萄球菌属和红球菌属。有几种细菌分离株对抗生素具有抗性。虽然尚未鉴定出厌氧细菌，但也记录到了它们的存在。真菌分离株通过其ITS序列进行分类。它们属于木霉属（肉座菌属）、青霉属、附球菌属、绿僵菌属（虫草属）和枝孢属。几种真菌产生了对标准细菌菌株具有抗菌作用的化合物。通过荧光显微镜、扫描电子显微镜和电子微探针分析获得了原生褐煤中存在微生物的证据。结果表明，微生物能够在低阶煤中长期存活。
