Colasante Claudia, Ellis Margrit, Ruppert Thomas, Voncken Frank
ZMBH, Heidelberg, Germany.
Proteomics. 2006 Jun;6(11):3275-93. doi: 10.1002/pmic.200500668.
Peroxisomes are present in nearly every eukaryotic cell and compartmentalize a wide range of important metabolic processes. Glycosomes of Kinetoplastid parasites are peroxisome-like organelles, characterized by the presence of the glycolytic pathway. The two replicating stages of Trypanosoma brucei brucei, the mammalian bloodstream form (BSF) and the insect (procyclic) form (PCF), undergo considerable adaptations in metabolism when switching between the two different hosts. These adaptations involve also substantial changes in the proteome of the glycosome. Comparative (non-quantitative) analysis of BSF and PCF glycosomes by nano LC-ESI-Q-TOF-MS resulted in the validation of known functional aspects of glycosomes and the identification of novel glycosomal constituents.
过氧化物酶体几乎存在于每一个真核细胞中，并将一系列重要的代谢过程分隔开来。动质体寄生虫的糖体是类似过氧化物酶体的细胞器，其特征是存在糖酵解途径。布氏布氏锥虫的两个复制阶段，即哺乳动物血液形式（BSF）和昆虫（前循环）形式（PCF），在两种不同宿主之间转换时，其代谢会经历相当大的适应性变化。这些适应性变化还涉及糖体蛋白质组的大量改变。通过纳米液相色谱-电喷雾电离-四极杆-飞行时间质谱对BSF和PCF糖体进行比较（非定量）分析，验证了糖体已知的功能方面，并鉴定出了新的糖体成分。
