Vascotto Carlo, Cesaratto Laura, D'Ambrosio Chiara, Scaloni Andrea, Avellini Claudio, Paron Igor, Baccarani Umberto, Adani Gian Luigi, Tiribelli Claudio, Quadrifoglio Franco, Tell Gianluca
Department of Biomedical Sciences and Technologies, University of Udine, Udine, Italy.
Proteomics. 2006 Jun;6(11):3455-65. doi: 10.1002/pmic.200500770.
Knowledge of early molecular events occurring upon ischemia/reperfusion (I/R) during liver transplantation (LT) is of great importance to improve the therapeutic intervention of surgical treatment. However, nowadays, few data are available on early protein targets of I/R injury. To identify these proteins, we used a differential proteomics approach in the characterization human liver biopsies during I/R upon LT. Analyses were performed on nine donor livers during LT. By using 2-DE and MALDI-TOF MS, we identified 36 proteins which resulted significantly altered upon I/R injury. The majority of these proteins are functionally involved in lipid and energy metabolism, in different metabolic pathways, in redox signalling and in oxidative-stress response. Our data represent the first global approach in the study of I/R injury in liver.
了解肝移植（LT）过程中缺血/再灌注（I/R）时发生的早期分子事件对于改善手术治疗的干预措施非常重要。然而，目前关于I/R损伤早期蛋白质靶点的数据很少。为了鉴定这些蛋白质，我们采用差异蛋白质组学方法对LT期间I/R时的人类肝脏活检样本进行表征。对LT期间的九个供体肝脏进行了分析。通过二维电泳（2-DE）和基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS），我们鉴定出36种在I/R损伤后发生显著变化的蛋白质。这些蛋白质中的大多数在脂质和能量代谢、不同代谢途径、氧化还原信号传导和氧化应激反应中发挥功能作用。我们的数据代表了对肝脏I/R损伤研究的首次全局方法。
