Ustinov J A, Loginov R J, Mattila P M, Nieminen V K, Suni J I, Häyry P J, Lautenschlager I T
Transplantation Laboratory, Fourth Department of Surgery, University of Helsinki, Finland.
Kidney Int. 1991 Nov;40(5):954-60. doi: 10.1038/ki.1991.300.
To study which structures of a kidney allograft are the main targets for cytomegalovirus (CMV), human glomerular epithelial and mesangial cells, as well as tubular epithelial and endothelial cells were isolated by steel meshes of different pore sizes and enzymatic treatments. The various cultured cell types were characterized by morphology and specific antibodies. Human CMV was inoculated onto cell monolayers using two different culture methods: conventional tissue culture and rapid shell vial culture. To analyze whether CMV had a direct effect on the immunologic properties of kidney parenchymal cells, MHC class I and class II antigen expression was estimated before and after the infection. CMV infected all kidney cells identically. All cells expressed class I strongly after the infection, but they were class I positive prior to infection. Class II antigens were not expressed on the cell surface either before or after the infection. In conclusion, human kidney cells of glomerular, tubular and vascular origin were all infected by CMV without any difference. CMV had no significant direct effects on the antigenic properties of the cells.
为研究肾移植器官的哪些结构是巨细胞病毒（CMV）的主要靶标，通过不同孔径的钢网和酶处理分离出人肾小球上皮细胞、系膜细胞以及肾小管上皮细胞和内皮细胞。通过形态学和特异性抗体对各种培养的细胞类型进行鉴定。使用两种不同的培养方法将人巨细胞病毒接种到细胞单层上：传统组织培养和快速空斑培养。为分析CMV是否对肾实质细胞的免疫特性有直接影响，在感染前后评估MHC I类和II类抗原表达。CMV对所有肾细胞的感染情况相同。感染后所有细胞均强烈表达I类抗原，但在感染前它们就是I类阳性。感染前后细胞表面均未表达II类抗原。总之，肾小球、肾小管和血管来源的人肾细胞均被CMV感染，无任何差异。CMV对细胞的抗原特性无显著直接影响。
