Heieren M H, Kim Y K, Balfour H H
Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis 55455.
Transplantation. 1988 Sep;46(3):426-32. doi: 10.1097/00007890-198809000-00019.
Evidence suggests that human cytomegalovirus is resident in the kidneys of seropositive donors at the time of transplantation, and CMV has been implicated in both glomerulonephritis and interstitial nephritis. In this study we assessed the interactions of CMV with two human renal cell types in culture: glomerular visceral epithelial cells (GVE) and renal tubular epithelial (RTE) cells. GVE permitted viral adsorption, penetration, nuclear translocation, and restricted viral transcription. However, early viral protein expression was not detectable by immunofluorescence and infectious virions were not produced. In contrast, retinoic acid-treated GVE permitted early viral protein expression and supported CMV replication. RTE also permitted viral adsorption and penetration. CMV-specific early proteins were readily observed by immunofluorescence, and CMV DNA replication was observed by DNA dot blot hybridization. Assays comparing viral yield with viral DNA synthesis indicated that RTE were capable of supporting persistent and prolonged viral expression without significant cell death for at least 55 days after infection. We believe that these findings should explain chronic viruria in individuals with symptomatic and asymptomatic CMV infection. In addition, GVE could also be a potential source of CMV transmission when altered by disease or transplantation.
有证据表明，在移植时，人类巨细胞病毒存在于血清反应阳性供体的肾脏中，并且巨细胞病毒与肾小球肾炎和间质性肾炎均有关联。在本研究中，我们评估了巨细胞病毒与培养中的两种人类肾细胞类型的相互作用：肾小球脏层上皮细胞（GVE）和肾小管上皮（RTE）细胞。GVE允许病毒吸附、穿透、核转位，并限制病毒转录。然而，通过免疫荧光无法检测到早期病毒蛋白表达，也未产生感染性病毒粒子。相比之下，经视黄酸处理的GVE允许早期病毒蛋白表达并支持巨细胞病毒复制。RTE也允许病毒吸附和穿透。通过免疫荧光很容易观察到巨细胞病毒特异性早期蛋白，通过DNA斑点印迹杂交观察到巨细胞病毒DNA复制。比较病毒产量与病毒DNA合成的测定表明，RTE能够支持持续和长时间的病毒表达，感染后至少55天内细胞无明显死亡。我们认为，这些发现应能解释有症状和无症状巨细胞病毒感染个体的慢性病毒尿。此外，当GVE因疾病或移植而发生改变时，它也可能是巨细胞病毒传播的潜在来源。
