大鼠脊髓和脑干培养星形胶质细胞上存在内皮素和血管加压素受体的电生理证据。

Electrophysiological evidence for the existence of receptors for endothelin and vasopressin on cultured astrocytes of rat spinal cord and brainstem.

作者信息

Hösli L, Hösli E, Lefkovits M, Wagner S

机构信息

Department of Physiology, University of Basel, Switzerland.

出版信息

Neurosci Lett. 1991 Oct 14;131(2):193-5. doi: 10.1016/0304-3940(91)90611-v.

DOI:10.1016/0304-3940(91)90611-v

PMID:1662342

Abstract

By means of intracellular recordings we have studied the actions of the vasoactive peptides endothelin-1 and -3 (ET-1, ET-3) and arginine vasopressin (AVP) on the membrane potential of astrocytes in explant cultures of rat spinal cord and brainstem. Addition of ET-1, ET-3 or AVP to the bathing solution at concentrations of 10(-8)-10(-6) M depolarized the membrane of the majority of glial cells studied. The AVP antagonist d(CH2)5(1), Tyr(Me)2, Arg8-vasopressin reversibly blocked the depolarizations by AVP. Our electrophysiological findings together with autoradiographic binding studies strongly suggest the existence of ET and AVP receptors on astrocytes.

摘要

通过细胞内记录，我们研究了血管活性肽内皮素 -1 和 -3（ET -1、ET -3）以及精氨酸加压素（AVP）对大鼠脊髓和脑干外植体培养物中星形胶质细胞膜电位的作用。以 10⁻⁸ - 10⁻⁶ M 的浓度将 ET -1、ET -3 或 AVP 添加到浴液中，使大多数所研究的神经胶质细胞膜发生去极化。AVP 拮抗剂 d(CH₂)₅(1)，Tyr(Me)₂，Arg⁸ - 加压素可逆转 AVP 引起的去极化。我们的电生理研究结果与放射自显影结合研究强烈表明星形胶质细胞上存在 ET 和 AVP 受体。