Hösli L, Hösli E, Käser H
Department of Physiology, University of Basel, Switzerland.
Neuroreport. 1993 Jun;4(6):679-82. doi: 10.1097/00001756-199306000-00019.
By means of combined immunohistochemical and auto-radiographic techniques we have studied the colocalization of cholinergic, adrenergic and peptidergic binding sites on astrocytes in explant cultures of rat spinal cord, brain stem and cerebellum. Many astrocytes which were immunostained by the monoclonal muscarinic receptor antibody M 35 were also intensely labelled by 3H-noradrenaline, the beta-adrenergic antagonist 3H-dihydroalprenolol and the peptides 125I-angiotensin II, 125I-neuropeptide Y and 3H-bradykinin. Electrophysiological studies demonstrating that muscarine, the adrenergic agonists noradrenaline and isoprenaline as well as angiotensin II, neuropeptide Y and bradykinin affect the membrane potential of the same astrocytes provide further evidence for the coexistence of cholinergic, adrenergic and peptidergic receptors on astrocytes.
通过联合免疫组织化学和放射自显影技术，我们研究了大鼠脊髓、脑干和小脑外植体培养物中星形胶质细胞上胆碱能、肾上腺素能和肽能结合位点的共定位情况。许多被单克隆毒蕈碱受体抗体M 35免疫染色的星形胶质细胞，也被3H - 去甲肾上腺素、β - 肾上腺素能拮抗剂3H - 二氢心得安以及肽类125I - 血管紧张素II、125I - 神经肽Y和3H - 缓激肽强烈标记。电生理研究表明，毒蕈碱、肾上腺素能激动剂去甲肾上腺素和异丙肾上腺素以及血管紧张素II、神经肽Y和缓激肽会影响同一星形胶质细胞的膜电位，这为星形胶质细胞上胆碱能、肾上腺素能和肽能受体的共存提供了进一步的证据。
