Avendaño-Herrera Ruben, Irgang Rute, Magariños Beatriz, Romalde Jesús L, Toranzo Alicia E
Departamento de Microbiología y Parasitología, Facultad de Biología and Instituto de Acuicultura, Universidad de Santiago, 15782 Santiago de Compostela, Spain.
Environ Microbiol. 2006 May;8(5):921-8. doi: 10.1111/j.1462-2920.2005.00981.x.
The survival of the fish pathogen Tenacibaculum maritimum in different seawater microcosms was investigated during 160 days. The persistence of culturable cells was greater in sterile than in natural seawater. Standard plate counts showed that T. maritimum survived in sterile seawater for more than 5 months at concentration around 10(3) cfu ml(-1). However, T. maritimum proved to be very labile in non-sterile seawater, rendering culturable cells no longer than 5 days. These results were confirmed when DNA-based methods were applied. Regardless of the microcosms used, epifluorescence microscopy counts remained at about 10(6) cells ml(-1) throughout the experiment, even though we can not distinguish T. maritimum in the case of non-sterile microcosms. Resuscitation assays with addition of fresh medium to non-sterile microcosms did not favour the recovery of T. maritimum on solid media. Although morphological changes from filamentous to spheres were observed after 3 days in the non-sterile microcosms, in the case of the sterile microcosms this change was observed at the sixth day. The biochemical, physiological, serological and genetic characteristics were unaffected in the sterile microcosms. The overall results contribute to a better understanding of the behaviour of T. maritimum in natural seawater and suggest that the aquatic bacterial population play an important role in the survival of this fish pathogen.
在160天的时间里，对鱼类病原菌海生黄杆菌（Tenacibaculum maritimum）在不同海水微宇宙中的存活情况进行了研究。可培养细胞在无菌海水中的持久性比在天然海水中更强。标准平板计数显示，海生黄杆菌在无菌海水中以约10³ cfu ml⁻¹的浓度存活了5个多月。然而，事实证明海生黄杆菌在非无菌海水中非常不稳定，可培养细胞存活时间不超过5天。当采用基于DNA的方法时，这些结果得到了证实。无论使用哪种微宇宙，在整个实验过程中，落射荧光显微镜计数均保持在约10⁶ 个细胞 ml⁻¹，尽管在非无菌微宇宙的情况下我们无法区分海生黄杆菌。向非无菌微宇宙中添加新鲜培养基进行复苏试验，不利于海生黄杆菌在固体培养基上的恢复。虽然在非无菌微宇宙中3天后观察到从丝状到球状的形态变化，但在无菌微宇宙中，这种变化在第6天才观察到。在无菌微宇宙中，其生化、生理、血清学和遗传特征未受影响。总体结果有助于更好地理解海生黄杆菌在天然海水中的行为，并表明水生细菌群体在这种鱼类病原菌的存活中起着重要作用。
