Yamaguchi Hiroyuki, Oshio Ichiro, Osaki Takako, Kurata Satoru, Yamamoto Yoshimasa, Kamiya Shigeru
Department of Basic Laboratory Sciences, Osaka University Graduate School of Medicine, Osaka, Japan.
Int J Exp Pathol. 2006 Apr;87(2):121-9. doi: 10.1111/j.0959-9673.2006.00468.x.
We examined a possible association between development of diabetes in non-obese diabetic (NOD) mice and dissemination of Chlamydia (Chlamydophila) pneumoniae from lung to peripheral blood. By real-time reverse transcription-polymerase chain reaction (RT-PCR) with primers for C. pneumoniae 16S rRNA, following multiple intranasal inoculations, we detected bacteria in lung in NOD mice with diabetes (38.5%) as well as Institute of Cancer Research, USA (ICR) mice (40%), but prevalence of bacteria in NOD mice without diabetes (pre-diabetic NOD mice and non-diabetic retired NOD mice) was very low (4.8%). The bacteria were only detected in peripheral blood mononuclear cells (PBMCs) cultured with hydrocortisone of the NOD mice with diabetes (53.8%). Results of immunostaining with fluorescein isothiocyanate-conjugated antichlamydia monoclonal antibody also showed the presence of bacterial antigens in the lungs and the PBMCs judged as positive by the RT-PCR. However, C. pneumoniae from cultured PBMCs of all NOD mice was undetected by cultivation method with inclusion-forming units assay. In addition, no influence of C. pneumoniae intranasal inoculation on development of diabetes in NOD mice was confirmed. Thus, the development of diabetes in NOD mouse appears to be one of critical factors for promoting the dissemination of C. pneumoniae from lung to peripheral blood.
我们研究了非肥胖型糖尿病（NOD）小鼠糖尿病的发生与肺炎衣原体从肺部扩散至外周血之间的可能关联。通过使用肺炎衣原体16S rRNA引物进行实时逆转录聚合酶链反应（RT-PCR），在多次鼻内接种后，我们在患有糖尿病的NOD小鼠（38.5%）以及美国癌症研究所（ICR）小鼠（40%）的肺部检测到了细菌，但在无糖尿病的NOD小鼠（糖尿病前期NOD小鼠和非糖尿病的老龄NOD小鼠）中细菌的检出率非常低（4.8%）。仅在使用氢化可的松培养的患有糖尿病的NOD小鼠的外周血单核细胞（PBMC）中检测到了细菌（53.8%）。用异硫氰酸荧光素偶联的抗衣原体单克隆抗体进行免疫染色的结果也显示，在RT-PCR判定为阳性的肺组织和PBMC中存在细菌抗原。然而，通过包涵体形成单位测定的培养方法未在所有NOD小鼠培养的PBMC中检测到肺炎衣原体。此外，未证实鼻内接种肺炎衣原体对NOD小鼠糖尿病发生有影响。因此，NOD小鼠糖尿病的发生似乎是促进肺炎衣原体从肺部扩散至外周血的关键因素之一。
