阿仑膦酸盐延缓了特立帕肽对去卵巢骨质疏松大鼠骨骼生物力学性能的改善作用

[Skeletal biomechanical properties improvement in ovariectomized osteoporotic rats of teriparatide was retarded by alendronate].

作者信息

Li Mei, Jiao Jie, Meng Xun-wu, Zhou Xue-ying, Xing Xiao-ping, Xia Wei-bo, Wang Ou, Jiang Yan, Liu Huai-cheng, Hu Ying-ying

机构信息

Department of Endocrinology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Science, Beijing 100730, China.

出版信息

Zhonghua Nei Ke Za Zhi. 2006 Feb;45(2):104-7.

PMID:16624115

Abstract

OBJECTIVE

To implore the effects of teriparatide (PTH) and alendronate (Alen) on skeletal biomechanical properties of ovariectomized (OVX) osteoporotic rats.

METHODS

70 female Wistar rats of 6 months were randomly divided into 7 groups: (1) Baseline: sacrificed at baseline; (2) OVXb: sacrificed in 6 weeks after OVX; (3) Sham operation; (4) OVXe: sacrificed 14 weeks after OVX; (5) PTH: 40 microg.kg(-1).d(-1); (6) Alen: 100 microg.kg(-1).d(-1); (7) A + P: PTH 40 microg.kg(-1).d(-1) and Alen 100 microg.kg(-1).d(-1). In group (5)-(7), different medicines were injected 5 times per week from 6th to 14th week after OVX. The cancellous biomechanical properties were measured with indentation test and the cortical properties were investigated with three-point bending test.

RESULTS

(1) The can load and can stiff of distal femur of OVXb were significantly lower than those of baseline (P < 0.01). It is indicated that osteoporotic rat models with impaired bone strength were established. (2) The can load and can stiff of distal femur of PTH [(36.3 +/- 9.2) N, (160.7 +/- 48.0) N/mm(2)], Alen [(42.7 +/- 13.0) N, (122.9 +/- 35.6) N/mm(2)] and A + P [(44.3 +/- 18.2) N, (105.2 +/- 58.4) N/mm(2)] groups were all higher than those of OVXe [(19.5 +/- 8.5) N, (83.2 +/- 37.7) N/mm(2), P < 0.001 or P < 0.01]. In femoral shaft, maximal load and elastic load in PTH, Alen and A + P groups were higher than those of OVXe (P < 0.001, P < 0.01 or P < 0.05). (3) Can stiff in distal femur was higher in PTH than that in Alen and A + P groups (P < 0.05). Elastic load, maximal load and energy absorption in femoral shaft were higher in PTH and A + P groups than those in Alen group (P < 0.05), and maximal stress was higher in PTH than that in A + P and Alen group (P < 0.05).

CONCLUSION

PTH and Alen were effective in improving the skeletal mechanical properties of OVX rats, but improvement in Alen and A + P was not so good as that in PTH group. It is indicated that anabolic effects of PTH was retarded by Alen's significant inhibition of bone turnover, therefore, PTH and Alen should not be utilized simultaneously.

摘要

目的

探讨特立帕肽（PTH）和阿仑膦酸盐（Alen）对去卵巢（OVX）骨质疏松大鼠骨骼生物力学性能的影响。

方法

将70只6月龄雌性Wistar大鼠随机分为7组：（1）基线组：在基线时处死；（2）OVXb组：去卵巢后6周处死；（3）假手术组；（4）OVXe组：去卵巢后14周处死；（5）PTH组：40μg·kg⁻¹·d⁻¹；（6）Alen组：100μg·kg⁻¹·d⁻¹；（7）A + P组：PTH 40μg·kg⁻¹·d⁻¹和Alen 100μg·kg⁻¹·d⁻¹。在第（5） - （7）组中，从去卵巢后第6周开始至第14周每周注射不同药物5次。用压痕试验测量松质骨生物力学性能，用三点弯曲试验研究皮质骨性能。

结果

（1）OVXb组大鼠股骨远端的骨小梁载荷和骨小梁硬度明显低于基线组（P < 0.01）。表明建立了骨强度受损的骨质疏松大鼠模型。（2）PTH组[（36.3±9.2）N，（160.7±48.0）N/mm²]、Alen组[（42.7±13.0）N，（122.9±35.6）N/mm²]和A + P组[（44.3±18.2）N，（105.2±58.4）N/mm²]股骨远端的骨小梁载荷和骨小梁硬度均高于OVXe组[（19.5±8.5）N，（83.2±37.7）N/mm²，P < 0.001或P < 0.01]。在股骨干，PTH组、Alen组和A + P组的最大载荷和弹性载荷高于OVXe组（P < 0.001、P < 0.01或P < 0.05）。（3）PTH组股骨远端的骨小梁硬度高于Alen组和A + P组（P < 0.05）。PTH组和A + P组股骨干的弹性载荷、最大载荷和能量吸收高于Alen组（P < 0.05），PTH组的最大应力高于A + P组和Alen组（P < 0.05）。