Scheideler Lutz, Rupp Frank, Wendel Hans P, Sathe Shila, Geis-Gerstorfer Jürgen
Department of Prosthodontics, Section Medical Materials and Technology, Center for Dental, Oral and Maxillofacial Medicine, University of Tübingen, Germany.
Dent Mater. 2007 Apr;23(4):469-78. doi: 10.1016/j.dental.2006.03.005. Epub 2006 Apr 19.
Coating of implant surfaces with biomolecules can influence basic host responses and enhance subsequent tissue integration. The biological factors have to be immobilized on the implant material. Human fibronectin (Fn) was used as a model protein and covalently coupled to titanium (Ti) surfaces via silanization and an anthraquinone linker. The impact on several aspects of initial host/biomaterial interactions (keratinocyte adhesion, platelet interactions and pellicle formation) was studied.
Coupling efficiency was characterized by immunological techniques. The effects of coupled Fn on initial host/biomaterial interactions were assessed. Cell adhesion and spreading were investigated by fluorescent staining, pellicle formation by an acoustic sensor system (quartz crystal microbalance with dissipation, QCM-D), and platelet adhesion as one parameter mediating the inflammatory response by scanning electron microscopy (SEM) and immunological assays.
Coupling efficiency was related to irradiation time used for photochemical coupling of the UV-activated anthraquinone to the silanized Ti surface. With an optimized protocol, the amount of Fn coupled to the surface could be almost doubled compared to standard dip-coating methods. On the anthraquinone-coupled Fn coatings, cell adhesion and spreading of human keratinocytes was significantly enhanced. Online detection of pellicle formation revealed strong reversibility of saliva protein adhesion on Fn coated surfaces compared to the pure Ti surface. Furthermore, the Fn coated Ti showed a low thrombogenicity.
This study suggests that anthraquinone-coupled biological coatings may be useful for biofunctionalization of Ti dental implants by enhancement of soft tissue re-integration (restoration of the epithelial seal) combined with diminished pellicle formation.
用生物分子对植入物表面进行涂层处理可影响宿主的基本反应并增强后续的组织整合。生物因子必须固定在植入材料上。人纤连蛋白（Fn）用作模型蛋白，并通过硅烷化和蒽醌连接体共价偶联到钛（Ti）表面。研究了其对初始宿主/生物材料相互作用的几个方面（角质形成细胞粘附、血小板相互作用和薄膜形成）的影响。
通过免疫技术表征偶联效率。评估偶联的Fn对初始宿主/生物材料相互作用的影响。通过荧光染色研究细胞粘附和铺展，通过声学传感器系统（耗散型石英晶体微天平，QCM-D）研究薄膜形成，通过扫描电子显微镜（SEM）和免疫测定法研究血小板粘附作为介导炎症反应的一个参数。
偶联效率与用于将紫外线激活的蒽醌光化学偶联到硅烷化Ti表面的照射时间有关。采用优化方案，与标准浸涂法相比，偶联到表面的Fn量几乎可增加一倍。在蒽醌偶联的Fn涂层上，人角质形成细胞的细胞粘附和铺展显著增强。与纯Ti表面相比，薄膜形成的在线检测显示唾液蛋白在Fn涂层表面的粘附具有很强的可逆性。此外，Fn涂层的Ti显示出低血栓形成性。
本研究表明，蒽醌偶联的生物涂层可通过增强软组织再整合（恢复上皮封闭）并减少薄膜形成，用于Ti牙科植入物的生物功能化。
