Prato Santina, Cannio Raffaele, Klenk Hans-Peter, Contursi Patrizia, Rossi Mosè, Bartolucci Simonetta
Dipartimento di Biologia Strutturale e Funzionale, Università degli Studi di Napoli Federico II, Complesso Universitario Monte S. Angelo, Via Cinthia, 80126, Naples, Italy.
Plasmid. 2006 Jul;56(1):35-45. doi: 10.1016/j.plasmid.2006.02.002. Epub 2006 Apr 19.
The plasmid pIT3 (4,967 bp) was isolated from the hyperthermophilic archaeon Sulfolobus solfataricus, strain IT3. The completely sequenced plasmid contains six open reading frames (ORFs), the largest (ORF915) spanning more than half of the plasmid and encoding a putative protein with significant similarity to the helicase domain of viral and plasmid primase proteins, as well as to the newly described archaeal primase-polymerase domain. A small ORF, (ORF80), located upstream of this putative polymerase, encodes a putative copy number control protein. Specific transcripts corresponding to the ORF80 and ORF915, were detected by Northern blot analyses, and their transcriptional start sites were determined by primer extension. Moreover, the transfer and the maintenance of the plasmid in other Sulfolobus strains were demonstrated to be effective and stable.
质粒pIT3（4967碱基对）从嗜热古菌嗜热栖热菌IT3菌株中分离得到。该完全测序的质粒包含六个开放阅读框（ORF），其中最大的（ORF915）跨越质粒的一半以上，编码一种推定蛋白，该蛋白与病毒和质粒引发酶蛋白的解旋酶结构域以及新描述的古菌引发酶 - 聚合酶结构域具有显著相似性。一个小的ORF（ORF80）位于该推定聚合酶的上游，编码一种推定的拷贝数控制蛋白。通过Northern印迹分析检测到与ORF80和ORF915对应的特异性转录本，并通过引物延伸确定了它们的转录起始位点。此外，该质粒在其他嗜热栖热菌菌株中的转移和维持被证明是有效且稳定的。
