Juzeniene Asta, Juzenas Petras, Bronshtein Irena, Vorobey Alexander, Moan Johan
Department of Radiation Biology, Institute for Cancer Research, The Norwegian Radium Hospital, N-0310 Montebello, Oslo, Norway.
J Photochem Photobiol B. 2006 Aug 1;84(2):161-6. doi: 10.1016/j.jphotobiol.2006.02.009. Epub 2006 Apr 18.
Cell survival was investigated after exposing cells in vitro to different temperatures before or after photodynamic therapy with 5-aminolevulinic acid. The photodynamic process was found to be temperature dependent. Cells exposed for 1h to 41 degrees C before light exposure or to 7 degrees C after light exposure showed decreased survival. Furthermore, the photobleaching rate of protoporphyrin IX in the cells was found to increase with increasing temperature during the light exposure. Thus, the photodynamic effect with 5-aminolevulinic acid may be enhanced by heating the tumour area before, and by cooling it immediately after the treatment.
在用5-氨基酮戊酸进行光动力治疗之前或之后，将体外培养的细胞暴露于不同温度下，研究细胞存活率。发现光动力过程与温度有关。在光照前暴露于41摄氏度1小时或光照后暴露于7摄氏度的细胞存活率降低。此外，发现细胞中原卟啉IX的光漂白率在光照期间随温度升高而增加。因此，在用5-氨基酮戊酸进行光动力治疗时，可通过在治疗前加热肿瘤区域和在治疗后立即冷却来增强光动力效应。
