Kristensen Torsten Nygaard, Sørensen Peter, Pedersen Kamilla Sofie, Kruhøffer Mogens, Loeschcke Volker
Aarhus Centre for Environmental Stress Research (ACES), Department of Ecology and Genetics, University of Aarhus, Denmark.
Genetics. 2006 Jul;173(3):1329-36. doi: 10.1534/genetics.105.054486. Epub 2006 Apr 19.
Genomewide gene expression patterns were investigated in inbred and noninbred Drosophila melanogaster lines under benign and stressful (high temperature) environmental conditions in a highly replicated experiment using Affymetrix gene chips. We found that both heat-shock protein and metabolism genes are strongly affected by temperature stress and that genes involved in metabolism are differentially expressed in inbred compared with noninbred lines, and that this effect is accentuated after heat stress exposure. Furthermore we show that inbreeding and temperature stress cause increased between-line variance in gene expression patterns. We conclude that inbreeding and environmental stress both independently and synergistically affect gene expression patterns. Interactions between inbreeding and the environment are often observed at the phenotypic level and our results reveal some of the genes that are involved at the individual gene level. Our observation of several metabolism genes being differentially expressed in inbred lines and more so after exposure to temperature stress, together with lower fitness in the investigated inbred lines, supports the hypothesis that superiority of heterozygous individuals partly derives from increased metabolic efficiency.
在一项使用Affymetrix基因芯片的高度重复实验中，研究了近交和非近交黑腹果蝇品系在良性和应激（高温）环境条件下的全基因组基因表达模式。我们发现，热休克蛋白和代谢基因均受到温度应激的强烈影响，与非近交品系相比，参与代谢的基因在近交品系中差异表达，并且这种影响在热应激暴露后会加剧。此外，我们表明近交和温度应激会导致品系间基因表达模式的差异增加。我们得出结论，近交和环境应激既独立又协同地影响基因表达模式。近交与环境之间的相互作用在表型水平上经常被观察到，我们的结果揭示了一些在个体基因水平上涉及的基因。我们观察到几个代谢基因在近交品系中差异表达，在暴露于温度应激后更是如此，再加上所研究的近交品系适应性较低，这支持了杂合个体的优势部分源于代谢效率提高的假设。
