Ali Bahy Ahmed, Huang Tian-Hua, Salem Halima-Hassan, Xie Qing-Dong
Research Center for Reproductive Medicine, Cell Biology and Medical Genetics Department, Shantou University Medical College, 22 Xinling Road, Shantou 515041, China.
Asian J Androl. 2006 May;8(3):273-9. doi: 10.1111/j.1745-7262.2006.00141.x.
To detect the expression of hepatitis B virus (HBV) genes (HB S and C genes) in early embryonic cells after introducing motile human sperm carrying HBV DNA into zona-free hamster oocytes via the in vitro fertilization (IVF) technique.
Human sperm-mediated HBV genes were delivered into zona-free hamster oocytes by the IVF method. Polymerase chain reaction (PCR) was used to detect HB S and pre-Core/Core (pre-C/C) coding genes both in one- and two-cell embryos. Reverse transcription-PCR (RT-PCR) analysis was used to study the expression of the two genes. Fluorescence in situ hybridization (FISH) analysis using the full-length HBV DNA as the hybridization probe was performed to confirm the integration of viral DNA in the host embryonic genome.
Both HB S and pre-C/C coding genes are present and transcribed in one- and two-cell embryos originated from hamster ova IVF with human spermatozoa carrying HBV DNA sequences.
Sperm-mediated HBV genes are able to replicate and express themselves in early embryonic cells. These results provide direct evidence that HBV DNA could transmit vertically to the next generation via the male germ line.
通过体外受精（IVF）技术将携带乙肝病毒（HBV）DNA的活动人类精子引入无透明带仓鼠卵母细胞后，检测早期胚胎细胞中乙肝病毒基因（HB S和C基因）的表达。
采用IVF方法将人精子介导的HBV基因导入无透明带仓鼠卵母细胞。聚合酶链反应（PCR）用于检测单细胞和双细胞胚胎中的HB S和前C/核心（pre-C/C）编码基因。逆转录-聚合酶链反应（RT-PCR）分析用于研究这两个基因的表达。使用全长HBV DNA作为杂交探针进行荧光原位杂交（FISH）分析，以确认病毒DNA在宿主胚胎基因组中的整合。
HB S和pre-C/C编码基因在源自携带HBV DNA序列的人类精子与仓鼠卵子IVF的单细胞和双细胞胚胎中均存在并转录。
精子介导的HBV基因能够在早期胚胎细胞中复制并表达。这些结果提供了直接证据，证明HBV DNA可通过雄性生殖系垂直传播给下一代。
