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Rapid bidirectional allele-specific PCR identification for triazine resistance in higher plants.

作者信息

Tian Xin, Darmency Henri

机构信息

Unité Mixte de Recherche Biologie et Gestion des Adventices, INRA, BP 86510, F-21065 Dijon, France.

出版信息

Pest Manag Sci. 2006 Jun;62(6):531-6. doi: 10.1002/ps.1197.

DOI:10.1002/ps.1197
PMID:16625683
Abstract

Triazine resistance is reported to be due to chloroplast herbicide target insensitivity in most species, and this is most often caused by a Ser(264)-Gly mutation at the D1 protein. In order to ascertain whether this mutation is really predominant amongst resistant plants, and also for gene flow studies, a rapid test is needed that allows the testing of large quantities of plants. Here a bidirectional allele-specific PCR (polymerase chain reaction) identification is proposed. The designed primers were shown to be universal in the three grass and three broadleaf species examined.

摘要

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