Pan Xue-Feng
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
Yi Chuan Xue Bao. 2006 Apr;33(4):373-80. doi: 10.1016/S0379-4172(06)60063-2.
Effects of degU32 (Hy), degR genes from Bacillus subtilis 168 and degQa gene from Bacillus amyloliquefaciens on Bacillus subtilis Ki-2-132 cell growth, sporulation and protease fermentation were investigated by introducing these genes into B. subtilis Ki-2-132 chromosome and/or cytoplasm. Although the genes come from different species and strains, they showed pleiotropic effects in B. subtilis Ki-2-132. B. subtilis Ki-2-132degU32 (Hy) showed increased protease production, and when cooperating with degQa either in plasmid or in chromosome, further altered cell growth, increased protease production and affected the spore formation in a glucose and dosage dependent manner. By contrast, degR did not significantly affect the protease productivity in degU32 (Hy) mutant, consisting with that DegR was used to stabilise DegU-phosphate, which in degU32 (Hy) strain no longer further amplify the DegU-phosphate effect.
通过将枯草芽孢杆菌168的degU32（Hy）、degR基因以及解淀粉芽孢杆菌的degQa基因导入枯草芽孢杆菌Ki-2-132的染色体和/或细胞质中，研究了这些基因对枯草芽孢杆菌Ki-2-132细胞生长、芽孢形成和蛋白酶发酵的影响。尽管这些基因来自不同的物种和菌株，但它们在枯草芽孢杆菌Ki-2-132中表现出多效性。枯草芽孢杆菌Ki-2-132degU32（Hy）的蛋白酶产量增加，并且当与degQa在质粒或染色体中协同作用时，会进一步改变细胞生长、增加蛋白酶产量，并以葡萄糖和剂量依赖的方式影响孢子形成。相比之下，degR对degU32（Hy）突变体中的蛋白酶生产力没有显著影响，这与DegR用于稳定磷酸化DegU一致，在degU32（Hy）菌株中不再进一步放大磷酸化DegU的作用。
