Olmos J, de Anda R, Ferrari E, Bolívar F, Valle F
Departamento de Microbiología Molecular, Universidad Nacional Autónoma de México, Cuernavaca.
Mol Gen Genet. 1997 Feb 20;253(5):562-7. doi: 10.1007/s004380050358.
The aprE gene of Bacillus subtilis codes for the serine alkaline protease known as subtilisin. Its expression is regulated by a complex network of activators and repressors that includes the products of hpr, degU and sinR. In order to understand the effect of these gene products on subtilisin expression, strains carrying combinations of the degU32(Hy), hpr2 and sinR null mutations, were constructed. We found that in all the genetic backgrounds tested, the sinR null mutation decreased aprE expression. Also, by measuring alkaline phosphatase synthesis and the formation of heat-resistant spores, as indicators of sporulation, we found that some of the mutant strains showed alterations in the sporulation process. These results suggest that these alterations are partially responsible for some of the observed changes in aprE expression.
枯草芽孢杆菌的aprE基因编码一种名为枯草杆菌蛋白酶的丝氨酸碱性蛋白酶。其表达受包括hpr、degU和sinR产物在内的激活因子和阻遏因子的复杂网络调控。为了了解这些基因产物对枯草杆菌蛋白酶表达的影响，构建了携带degU32(Hy)、hpr2和sinR无效突变组合的菌株。我们发现，在所有测试的遗传背景中，sinR无效突变均降低了aprE的表达。此外，通过测量碱性磷酸酶合成和耐热孢子的形成作为芽孢形成的指标，我们发现一些突变菌株在芽孢形成过程中出现了改变。这些结果表明，这些改变部分导致了观察到的aprE表达变化。
