Xu Chengyang, Vinegoni Claudio, Ralston Tyler S, Luo Wei, Tan Wei, Boppart Stephen A
Biophotonics Imaging Laboratory, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, 405 North Mathews Avenue, Urbana, Illinois 61801, USA.
Opt Lett. 2006 Apr 15;31(8):1079-81. doi: 10.1364/ol.31.001079.
The spectroscopic content within optical coherence tomography (OCT) data can provide a wealth of information. Spectroscopic OCT methods are frequently limited by time-frequency trade-offs that limit high spectral and spatial resolution simultaneously. We present spectroscopic spectral-domain optical coherence microscopy performed with a multimodality microscope. Restricting the spatial extent of the signal by using high-numerical-aperture optics makes high-resolution spectroscopic information accessible, facilitated with spectral-domain detection. Simultaneous acquisition of multiphoton microscopy images is used to validate tissue structure and localization of nuclei within individual cells.
光学相干断层扫描（OCT）数据中的光谱内容可以提供丰富的信息。光谱OCT方法常常受到时频权衡的限制，这种权衡会同时限制高光谱分辨率和高空间分辨率。我们展示了使用多模态显微镜进行的光谱频域光学相干显微镜检查。通过使用高数值孔径光学器件来限制信号的空间范围，使得高分辨率光谱信息变得可获取，并借助频域检测得以实现。同时采集多光子显微镜图像用于验证组织结构以及单个细胞内核的定位。
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