Chelliyil Robin G, Ralston Tyler S, Marks Daniel L, Boppart Stephen A
University of Illinois at Urbana-Champaign, Department of Electrical and Computer Engineering, Beckman Institute for Advanced Science and Technology, Biophotonics Imaging Laboratory, Urbana, Illinois 61801, USA.
J Biomed Opt. 2008 Jul-Aug;13(4):044013. doi: 10.1117/1.2960018.
Optical coherence microscopy (OCM) is an interferometric technique that combines principles of confocal microscopy and optical coherence tomography (OCT) to obtain high-resolution en face images. Axial and lateral resolutions of several microns can be achieved using OCM depending on the numerical aperture (NA) of the objective and sample properties. We address the computational complexity that is inherent in spectral-domain OCM systems that limits its real-time capability as a microscope. An architecture that will improve the efficiency of the computation involved is presented. Currently, spectral-domain OCM images are obtained by individually taking the Fourier transform of each axial scan in cross-sectional frames and computationally slicing them to generate en face images. The real-time architecture presented here relies on the fact that only one Fourier domain point of a given axial scan needs to be computed rather than computing all the Fourier domain points, which can frequently require a significant amount of time to compute. This new realization has been shown to reduce the processing time to obtain the en face OCM images by a factor of 30.
光学相干显微镜（OCM）是一种干涉技术，它结合了共聚焦显微镜和光学相干断层扫描（OCT）的原理，以获取高分辨率的正面图像。根据物镜的数值孔径（NA）和样品特性，使用OCM可以实现几微米的轴向和横向分辨率。我们解决了光谱域OCM系统中固有的计算复杂性问题，这种复杂性限制了其作为显微镜的实时能力。本文提出了一种能提高相关计算效率的架构。目前，光谱域OCM图像是通过对横截面帧中的每个轴向扫描分别进行傅里叶变换，并通过计算切片来生成正面图像的。这里提出的实时架构基于这样一个事实，即对于给定的轴向扫描，只需要计算一个傅里叶域点，而不是计算所有的傅里叶域点，后者通常需要大量时间来计算。这种新方法已被证明能将获取正面OCM图像的处理时间缩短30倍。
