Yoon Changsik, Qi Yue, Mestre Humberto, Canavesi Cristina, Marola Olivia J, Cogliati Andrea, Nedergaard Maiken, Libby Richard T, Rolland Jannick P
The Institute of Optics, University of Rochester, Wilmot Building, Rochester, New York 14627, USA.
Department of Biomedical Engineering, University of Rochester, Robert B. Goergen Hall, Rochester, New York 14627, USA.
Biomed Opt Express. 2019 Nov 14;10(12):6242-6257. doi: 10.1364/BOE.10.006242. eCollection 2019 Dec 1.
We report on the development of fluorescence Gabor domain optical coherence microscopy (Fluo GD-OCM), a combination of GD-OCM with laser scanning confocal fluorescence microscopy (LSCFM) for synchronous micro-structural and fluorescence imaging. The dynamic focusing capability of GD-OCM provided the adaptive illumination environment for both modalities without any mechanical movement. Using Fluo GD-OCM, we imaged DsRed-expressing cells in the brain of a transgenic mouse, as well as Cy3-labeled ganglion cells and Cy3-labeled astrocytes from a mouse retina. The self-registration of images taken by the two different imaging modalities showed the potential for a correlative study of subjects and double identification of the target.
我们报道了荧光伽柏域光学相干显微镜（Fluo GD-OCM）的发展情况，它是将伽柏域光学相干显微镜（GD-OCM）与激光扫描共聚焦荧光显微镜（LSCFM）相结合，用于同步微观结构和荧光成像。GD-OCM的动态聚焦能力为这两种成像方式提供了自适应照明环境，且无需任何机械移动。使用Fluo GD-OCM，我们对转基因小鼠大脑中表达红色荧光蛋白的细胞，以及来自小鼠视网膜的Cy3标记的神经节细胞和Cy3标记的星形胶质细胞进行了成像。由两种不同成像方式拍摄的图像的自动配准显示了对研究对象进行相关研究和对目标进行双重识别的潜力。
