Truyen U, Wilhelm S, Genzow M, Schagemann G
Institut für Tierhygiene und Offentliches Veterinärwesen, Veterinärmedizinische Fakultät, Universität Leipzig, Leipzig, Germany.
J Vet Med B Infect Dis Vet Public Health. 2006 Mar;53(2):68-74. doi: 10.1111/j.1439-0450.2006.00913.x.
Porcine reproductive and respiratory syndrome virus (PRRSV) is a major pathogen of swine. Due to genetic variation between the European and the US genotype as well as within both genotypes detection of PRRSV is a diagnostic challenge. This paper reports on a ring test to compare different established reverse transcriptase polymerase chain reaction methods applied routinely in 16 different laboratories in Germany. Three different sets of samples were sent to the laboratories which were to be analysed as follows: (i) basis package: detection of PRRS (yes/no); (ii) differentiation package I: differentiation of EU and US genotypes; and (iii) differentiation package II: differentiation of EU field isolates and EU vaccine strain. A total of 80% of the samples of the basic package were analysed correctly, the analysis of the differentiation package I revealed 61.82% correctly tested samples and the two laboratories that analysed the differentiation package II showed only one correct result. The ring test showed that the majority of incorrect diagnoses were false-negative results.
猪繁殖与呼吸综合征病毒（PRRSV）是猪的主要病原体。由于欧洲基因型和美国基因型之间以及两种基因型内部存在基因变异，PRRSV的检测是一项诊断挑战。本文报道了一项环式检验，以比较德国16个不同实验室常规应用的不同成熟的逆转录聚合酶链反应方法。向各实验室发送了三组不同的样本，分析要求如下：（i）基础套餐：检测PRRS（是/否）；（ii）鉴别套餐I：区分欧洲和美国基因型；（iii）鉴别套餐II：区分欧洲野毒株和欧洲疫苗株。基础套餐样本的总体分析正确率为80%，鉴别套餐I的分析显示样本检测正确率为61.82%，而分析鉴别套餐II的两个实验室仅得出一个正确结果。环式检验表明，大多数错误诊断为假阴性结果。
