Harding John C S, Baker Crissie, Rhodes Carrie, McIntosh Kathleen A, Bonneau Martin
Department of Large Animal Clinical Science, Western College of Veterinary Medicine, Saskatchewan, Canada.
Can J Vet Res. 2009 Jan;73(1):7-14.
Two laboratory studies involving 11 laboratories were undertaken to assess the performance of North American Porcine circovirus-2 (PCV-2) polymerase chain reaction (PCR) assays. Laboratories received identical submissions containing randomly coded positive and negative control samples, and serially diluted PCV-2-spiked samples. In study 1 and 2, respectively, spiked samples contained measured amounts of PCV-2 virus or DNA. All but 1 assay detected DNA in the most concentrated spiked sample. There were no statistical differences in the proportion of positive or negative samples reported by quantitative (n = 7) versus non-quantitative (n = 6) assays. Across both studies, the false positive rate was 17% (4 out of 23), and 17% (2 out of 12) of assays cross-reacted with PCV-1. The most sensitive assay detected PCV-2 DNA levels about 100 000 times lower the least sensitive assay. This study demonstrated that the PCR assays available in North American diagnostic labs vary considerably in their detection limits and quantification.
开展了两项涉及11个实验室的实验室研究，以评估北美猪圆环病毒2型（PCV - 2）聚合酶链反应（PCR）检测方法的性能。各实验室收到了相同的样本，其中包括随机编码的阳性和阴性对照样本，以及经系列稀释的PCV - 2加标样本。在研究1和研究2中，加标样本分别含有定量的PCV - 2病毒或DNA。除1种检测方法外，其他所有检测方法均在浓度最高的加标样本中检测到了DNA。定量检测方法（n = 7）和非定量检测方法（n = 6）报告的阳性或阴性样本比例无统计学差异。在两项研究中，假阳性率为17%（23个样本中有4个），17%（12种检测方法中有2种）的检测方法与PCV - 1发生交叉反应。最灵敏的检测方法检测到的PCV - 2 DNA水平比最不灵敏的检测方法低约100000倍。这项研究表明，北美诊断实验室现有的PCR检测方法在检测限和定量方面差异很大。
