Ma Dong-chao, Yang Zhi-jian, Wang Wei, Xu Shun-lin, Zhang Yu-Qing, Zhou Fang, Chen Bo, Xu Zhao-qiang, Cao Ke-jiang, Ma Wen-zhu
Department of Cardiology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Zhonghua Xin Xue Guan Bing Za Zhi. 2006 Feb;34(2):119-22.
To evaluate the impact of combined therapy with transplanting bone marrow-derived mesenchymal stem cells (BM-MSCs) via noninfarct-relative artery and hepatocyte growth factor (HGF) in a porcine myocardial infarction (MI) model.
BM-MSCs were obtained from pig bone marrow, expanded in vitro with a purity of > 50%. MI was induced by ligating the distal left anterior descending artery in pigs. Eighteen animals received BM-MSCs cells (5 x 10(6)/ml, n = 6), BM-MSCs cells (5 x 10(6)/ml) plus HGF (4 x 10(9) pfu, n = 6) or equal volume culture medium (IMDM) via non-infarct-related artery at four weeks after MI. Gated myocardial perfusion imaging and coronary angiography were performed before and four weeks after transplantations. Histological examination was also performed 4 weeks after transplantation.
LVEF measured by gated myocardial perfusion imaging was similar among groups before transplantation and significantly increased in BM-MSCs (45 +/- 3 vs. 34 +/- 2%, P < 0.05) or BM-MSCs + HGF (46 +/- 6 vs. 34 +/- 3%, P < 0.05) treated animals while remained unchanged in IMDM (30 +/- 3 vs. 32 +/- 2%) treated animals 4 weeks post transplantation. Similarly, capillary density was also significantly higher and myocardial perfusion defect scores significantly decreased in BM-MSCs or BM-MSCs + HGF treated hearts than that in IMDM treated hearts. However, all these changes were similar between BM-MSCs and BM-MSCs + HGF groups. Rentrop score was similar before and 4 weeks after transplantation among various groups.
HGF in combination with BM-MSCs transplantation did not enhance the cardiac repair effects of BM-MSCs transplantation alone and BM-MSCs transplantation did not improve collateral circulation in this model.
在猪心肌梗死（MI）模型中评估经非梗死相关动脉移植骨髓间充质干细胞（BM-MSCs）与肝细胞生长因子（HGF）联合治疗的效果。
从猪骨髓中获取BM-MSCs，体外扩增后纯度>50%。通过结扎猪左前降支远端诱导MI。18只动物在MI后4周经非梗死相关动脉接受BM-MSCs细胞（5×10⁶/ml，n = 6）、BM-MSCs细胞（5×10⁶/ml）加HGF（4×10⁹ pfu，n = 6）或等体积培养基（IMDM）。在移植前和移植后4周进行门控心肌灌注成像和冠状动脉造影。移植后4周也进行组织学检查。
移植前各组间通过门控心肌灌注成像测量的左室射血分数（LVEF）相似，移植后4周，接受BM-MSCs（45±3%对34±2%，P<0.05）或BM-MSCs+HGF（46±6%对34±3%，P<0.05）治疗的动物LVEF显著增加，而接受IMDM（30±3%对32±2%）治疗的动物LVEF无变化。同样，BM-MSCs或BM-MSCs+HGF治疗的心脏中毛细血管密度也显著更高，心肌灌注缺损评分显著低于IMDM治疗的心脏。然而，BM-MSCs组和BM-MSCs+HGF组之间所有这些变化相似。各组移植前和移植后4周的Rentrop评分相似。
在该模型中，HGF与BM-MSCs移植联合并未增强单独BM-MSCs移植的心脏修复效果，且BM-MSCs移植未改善侧支循环。
