Moon Joong Ho, McDaniel William, Hancock Lawrence F
Nomadics, Inc., 215 First St., Suite 104, Cambridge, MA 02142, USA.
J Colloid Interface Sci. 2006 Aug 1;300(1):117-22. doi: 10.1016/j.jcis.2006.03.063. Epub 2006 Apr 3.
Fabrication, characterization, and application of poly(phenylene ethynylene) (PPE)/silica composite particles are described. PPE is a class of conjugated polymers, which has been used for various sensory materials. However, its hydrophobic nature makes its application difficult in the aqueous phase, especially for biological substance detection. In this report, we utilized non-aqueous soluble PPE, 15 nm of colloidal silica particles, and aminosilane to fabricate a biosensory platform. The resulting composite showed high aqueous compatibility, large surface area, high quantum efficiency, and versatile chemical modification including oligonucleotide coupling. By monitoring the fluorescence quenching of PPE, we could detect a quencher-labeled target oligonucleotide specifically. Stern-Volmer (SV) analysis showed different accessibility of fluorophores (PPE) to a quencher labeled target oligonucleotide. The accessibility of fluorophores and SV constant are determined to be 0.54 and 4.2 x 10(7)M(-1), respectively, from a modified SV plot. This method will broaden the capability of conjugated polymers for the sensitive detection of biological substances.
本文描述了聚亚苯基乙炔（PPE）/二氧化硅复合颗粒的制备、表征及应用。PPE是一类共轭聚合物，已被用于各种传感材料。然而，其疏水性使其在水相中应用困难，尤其是用于生物物质检测。在本报告中，我们利用非水可溶性PPE、15纳米的胶体二氧化硅颗粒和氨基硅烷构建了一个生物传感平台。所得复合材料具有高水相兼容性、大表面积、高量子效率以及包括寡核苷酸偶联在内的多种化学修饰。通过监测PPE的荧光猝灭，我们能够特异性地检测淬灭剂标记的靶寡核苷酸。斯特恩-沃尔默（SV）分析表明荧光团（PPE）对淬灭剂标记的靶寡核苷酸具有不同的可及性。根据修正后的SV图，荧光团的可及性和SV常数分别确定为0.54和4.2×10⁷M⁻¹。该方法将拓宽共轭聚合物对生物物质的灵敏检测能力。
