Guangdong Province Key Laboratory of Chemical Biology, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, PR China.
Langmuir. 2010 Feb 2;26(3):1528-32. doi: 10.1021/la903970t.
We investigated the quenching properties of poly(phenylene ethynylene) with anionic alkoxyl sulfonate side groups, PPE-SO3, by tris(2,2-bipyridyl)dichlororuthenium(II) (Rubpy)-doped silica nanoparticles (SiNPs) in water, methanol, and the surfactant Triton X-100 aqueous solution. SiNPs demonstrated hyper-efficient quenching characterized by the Stern-Volmer constants in the range of 10(9)-10(10) M(-1), which is about 100-10,000 times more efficient compared to the Rubpy dye. More importantly, quenching by SiNPs was found to be more efficient when the polymer existed as a nonaggregated state, such as in methanol solution and in the surfactant solution. Investigations on confocal fluorescence images and time-resolved fluorescence decays were carried out to study the quenching mechanism.
我们研究了带有阴离子烷氧基磺酸盐侧基的聚（苯乙炔）（PPE-SO3）在水中、甲醇中和表面活性剂 Triton X-100 水溶液中掺杂三（2,2-联吡啶）二氯钌（II）（Rubpy）的硅纳米粒子（SiNPs）的猝灭性能。SiNPs 表现出超高效猝灭特性，其 Stern-Volmer 常数范围为 10(9)-10(10) M(-1)，与 Rubpy 染料相比，效率提高了 100-10000 倍。更重要的是，当聚合物处于非聚集状态时，如在甲醇溶液中和表面活性剂溶液中，SiNPs 的猝灭效率更高。通过共聚焦荧光图像和时间分辨荧光衰减研究了猝灭机制。
