Pap E, Falus A, Mihályi D, Borck H, Diel F, Pállinger E
Department of Genetics, Cell and Immunobiology, Semmelweis University, Budapest, Hungary.
Placenta. 2007 Feb-Mar;28(2-3):239-44. doi: 10.1016/j.placenta.2006.02.010. Epub 2006 Apr 19.
Successful pregnancy is closely related to polarization toward a Th2 type immune response. As histamine is known to initiate Th2 dominance during inflammatory processes we raised the question whether histamine has any effect on the actual tuning of proper cytokine balance for the proceeding of the gestation. Histamine has multiple functions in the process of pregnancy, different studies have shown the direct and/or indirect presence of histamine action in the placenta as well. As HDC is the unique histamine producing enzyme in eukaryotes, we used HDC (so endogenous histamine)-deficient knockout mice as reliable model for studying histamine-related processes in vivo. We examined the placental histamine content and the expression of histamine receptors and Th1/Th2/Th3 type cytokines in the placenta. We showed for the first time the influence of histamine on the orchestrated regulation of placental cytokine expression. In the absence of local histamine the cytokine balance is shifted toward Th1 types at the maternal-placental interface, threatening pregnancy. We also measured splenic lymphocyte subpopulation ratios in pregnant and non-pregnant mice and found that in pregnancy they are independent of the presence of histamine.
成功妊娠与向Th2型免疫反应的极化密切相关。由于已知组胺在炎症过程中启动Th2优势，我们提出了一个问题，即组胺对妊娠进程中适当细胞因子平衡的实际调节是否有任何影响。组胺在妊娠过程中具有多种功能，不同研究表明胎盘也存在组胺作用的直接和/或间接证据。由于组胺脱羧酶（HDC）是真核生物中唯一产生组胺的酶，我们使用HDC（即内源性组胺）缺陷型敲除小鼠作为研究体内组胺相关过程的可靠模型。我们检测了胎盘组胺含量以及胎盘组胺受体和Th1/Th2/Th3型细胞因子的表达。我们首次展示了组胺对胎盘细胞因子表达的协调调节的影响。在缺乏局部组胺的情况下，母胎界面处的细胞因子平衡向Th1型转变，危及妊娠。我们还测量了妊娠和未妊娠小鼠的脾淋巴细胞亚群比例，发现妊娠时它们与组胺的存在无关。
