利比亚腹泻和非腹泻儿童中肠致病性大肠杆菌（EPEC）和肠集聚性大肠杆菌（EAEC）的表型及基因特征分析

Phenotypic and genetic characterization of enteropathogenic Escherichia coli (EPEC) and entero-aggregative E. coli (EAEC) from diarrhoeal and non-diarrhoeal children in Libya.

作者信息

Dow M A, Tóth I, Malik A, Herpay M, Nógrády N, Ghenghesh K S, Nagy B

机构信息

Veterinary Medical Research Institute of the Hungarian Academy of Sciences, Budapest, Hungary.

出版信息

Comp Immunol Microbiol Infect Dis. 2006 Mar-May;29(2-3):100-13. doi: 10.1016/j.cimid.2006.01.005. Epub 2006 Apr 19.

DOI:10.1016/j.cimid.2006.01.005

PMID:16626804

Abstract

A total of 50 Escherichia coli strains isolated in a Libyan hospital (20 from children with diarrhoea and 30 from healthy children) were investigated for their pathotypes and virulence traits. Altogether nine eae-positive (enteropathogenic E. coli, EPEC) and nine aggR-positive (entero-aggregative E. coli, EAEC) strains were identified. Significantly (P=0.001) more EPEC strains were identified from diarrhoeal patients (n=8) than from healthy controls (n=1), while six EAEC strains were identified from diarrhoeal and three from healthy children. Typical (eae(+), EAF(+), bfp(+)) EPEC strains (n=6) belonged to classical EPEC serogroups O55, O114, O127 and showed localized adherence on Hela cells. EAEC strains revealed genetic heterogeneity but uniformly adhered to HeLa cultures in an entero-aggregative adherence pattern. Antibiotic resistance frequently, characterized the strains. Sixty-eight percentage of the strains were resistant against at least one antibiotic and 30% harbored a class 1 integron independently of their clinical background. This is the first report from North Africa demonstrating the significance of EPEC and EAEC.

摘要

对利比亚一家医院分离出的50株大肠杆菌菌株（20株来自腹泻儿童，30株来自健康儿童）进行了致病型和毒力特征研究。共鉴定出9株eae阳性（肠致病性大肠杆菌，EPEC）和9株aggR阳性（肠集聚性大肠杆菌，EAEC）菌株。腹泻患者中鉴定出的EPEC菌株（n = 8）显著多于健康对照（n = 1）（P = 0.001），腹泻儿童中鉴定出6株EAEC菌株，健康儿童中鉴定出3株。典型的（eae(+)、EAF(+)、bfp(+)）EPEC菌株（n = 6）属于经典EPEC血清型O55、O114、O127，在Hela细胞上表现为局部黏附。EAEC菌株显示出遗传异质性，但均以肠集聚性黏附模式黏附于Hela培养物。这些菌株常常具有抗生素抗性。68%的菌株对至少一种抗生素耐药，30%携带1类整合子，且与它们的临床背景无关。这是来自北非的首份报告，证明了EPEC和EAEC的重要性。