利比亚致泻性大肠杆菌的分子特征。
Molecular characterization of diarrheagenic Escherichia coli from Libya.
机构信息
Department of Botany, Faculty of Science, Cairo University, Cairo, Egypt.
出版信息
Am J Trop Med Hyg. 2012 May;86(5):866-71. doi: 10.4269/ajtmh.2012.11-0330.
Diarrheagenic Escherichia coli (DEC) are important enteric pathogens that cause a wide variety of gastrointestinal diseases, particularly in children. Escherichia coli isolates cultured from 243 diarrheal stool samples obtained from Libyan children and 50 water samples were screened by polymerase chain reaction (PCR) for genes characteristic of enteroaggregative E. coli (EAEC), enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC), enterohemorrhagic E. coli (EHEC), and enteroinvasive E. coli (EIEC). The DEC were detected in 21 (8.6%) children with diarrhea; 10 (4.1%) cases were identified as EAEC, 3 (1.2%) as EPEC, and 8 (3.3%) were ETEC; EHEC, and EIEC were not detected. All DEC were grouped phylogenetically by PCR with the majority (> 70%) identified as phylogenetic groups A and B1. The EAEC isolates were also tested for eight genes associated with virulence using PCR. Multi-virulence (≥ 3 virulence factors) was found in 50% of EAEC isolates. Isolated EAEC possessed different virulence traits and belonged to different phylogenetic groups indicating their heterogeneity.
肠致病性大肠杆菌（DEC）是重要的肠道病原体，可引起多种胃肠道疾病，尤其是在儿童中。通过聚合酶链反应（PCR）对从 243 份利比亚儿童腹泻粪便样本和 50 份水样中培养的大肠杆菌分离株进行筛选，以检测肠聚集性大肠杆菌（EAEC）、肠致病性大肠杆菌（EPEC）、肠毒性大肠杆菌（ETEC）、肠出血性大肠杆菌（EHEC）和肠侵袭性大肠杆菌（EIEC）的特征基因。在 21 名腹泻儿童（8.6%）中检测到 DEC；10 例（4.1%）为 EAEC，3 例（1.2%）为 EPEC，8 例（3.3%）为 ETEC；未检测到 EHEC 和 EIEC。所有 DEC 均通过 PCR 进行了系统发育分组，其中大多数（>70%）鉴定为 A 和 B1 组。还使用 PCR 对 EAEC 分离株的 8 个与毒力相关的基因进行了检测。50%的 EAEC 分离株存在多毒力（≥3 种毒力因子）。分离的 EAEC 具有不同的毒力特征，并属于不同的系统发育群，表明其具有异质性。
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