Coombs J, van der List D, Wang G-Y, Chalupa L M
Section of Neurobiology, Physiology and Behavior, College of Biological Sciences, University of California, One Shields Avenue, Davis, CA 95616, USA.
Neuroscience. 2006 Jun 19;140(1):123-36. doi: 10.1016/j.neuroscience.2006.02.079. Epub 2006 Apr 19.
The mouse retina offers an increasingly valuable model for vision research given the possibilities for genetic manipulation. Here we assess how the structural properties of mouse retinal ganglion cells relate to the stratification pattern of the dendrites of these neurons within the inner plexiform layer. For this purpose, we used 14 morphological measures to classify mouse retinal ganglion cells parametrically into different clusters. Retinal ganglion cells were labeled in one of three ways: Lucifer Yellow injection, 'DiOlistics' or transgenic expression of yellow fluorescent protein. The resulting analysis of 182 cells revealed 10 clusters of monostratified cells, with dendrites confined to either On or Off sublaminae of the inner plexiform layer, and four clusters of bistratified cells, dendrites spanning the On and Off sublaminae. We also sought to establish how these parametrically identified retinal ganglion cell clusters relate to cell types identified previously on the basis of immunocytochemical staining and the expression of yellow fluorescent protein. Cells labeled with an antibody against melanopsin were found to be located within a single cluster, while those labeled with the SMI-32 antibody were in four different clusters. Yellow fluorescent protein expressing cells were distributed within 13 of the 14 clusters identified here, which demonstrates that yellow fluorescent protein expression is a useful method for labeling virtually the entire population of mouse retinal ganglion cells. Collectively, these findings provide a valuable baseline for future studies dealing with the effects of genetic mutations on the morphological development of these neurons.
鉴于基因操作的可能性，小鼠视网膜为视觉研究提供了一个越来越有价值的模型。在这里，我们评估小鼠视网膜神经节细胞的结构特性如何与这些神经元在内网状层内树突的分层模式相关。为此，我们使用14种形态学测量方法将小鼠视网膜神经节细胞参数化分类为不同的簇。视网膜神经节细胞通过三种方式之一进行标记：注射路西法黄、“DiOlistics”或黄色荧光蛋白的转基因表达。对182个细胞的分析结果显示，有10个单分层细胞簇，其树突局限于内网状层的On或Off亚层，还有4个双分层细胞簇，其树突跨越On和Off亚层。我们还试图确定这些通过参数识别的视网膜神经节细胞簇如何与先前基于免疫细胞化学染色和黄色荧光蛋白表达确定的细胞类型相关。发现用抗黑视蛋白抗体标记的细胞位于单个簇内，而用SMI-32抗体标记的细胞则分布在四个不同的簇中。表达黄色荧光蛋白的细胞分布在此处确定的14个簇中的13个簇内，这表明黄色荧光蛋白表达是标记几乎整个小鼠视网膜神经节细胞群体的一种有用方法。总的来说，这些发现为未来研究基因突变对这些神经元形态发育的影响提供了有价值的基线。
