Sarfati Jacqueline, Monod Michael, Recco Paulette, Sulahian Annie, Pinel Claudine, Candolfi Ermanno, Fontaine Thierry, Debeaupuis Jean-Paul, Tabouret Marc, Latgé Jean-Paul
Unité des Aspergillus, Institut Pasteur, 75015 Paris, France.
Diagn Microbiol Infect Dis. 2006 Aug;55(4):279-91. doi: 10.1016/j.diagmicrobio.2006.02.002. Epub 2006 Apr 19.
Eight recombinant proteins and purified galactomannan of Aspergillus fumigatus were tested by enzyme-linked immunosorbent assay to quantify the anti-Aspergillus antibodies in sera of patients with aspergilloma, allergic bronchopulmonary aspergillosis (ABPA), and invasive aspergillosis (IA). In spite of the variability observed in the immune responses of individual patients, quantification of the antibody titers against the 18-kDa ribonuclease (RNU), the 360-kDa catalase (CAT), and the 88-kDa dipeptidylpeptidase V (DPPV) was useful for the diagnosis of aspergilloma and ABPA. Differential diagnosis of ABPA was even possible among cystic fibrosis as well as noncystic fibrosis patients. In the group of immunocompromised patients with IA, no antibody response was mounted in response to the Aspergillus infection in any of the patients. Interestingly, about half of the patients with proven IA came to the hospital with high titers of anti-Aspergillus antibodies, suggesting that they were infected upon entry to the hospital. These results suggest that recombinant RNU, CAT, and DPPV have a great potential in the serodiagnosis of all forms of aspergillosis in the immunocompromised and immunocompetent patient.
通过酶联免疫吸附测定法对8种重组蛋白和烟曲霉纯化的半乳甘露聚糖进行检测，以定量分析曲菌球、变应性支气管肺曲霉病（ABPA）和侵袭性曲霉病（IA）患者血清中的抗曲霉抗体。尽管个体患者的免疫反应存在差异，但针对18 kDa核糖核酸酶（RNU）、360 kDa过氧化氢酶（CAT）和88 kDa二肽基肽酶V（DPPV）的抗体滴度定量分析对曲菌球和ABPA的诊断很有用。在囊性纤维化以及非囊性纤维化患者中，甚至有可能对ABPA进行鉴别诊断。在免疫功能低下的IA患者组中，任何患者均未对曲霉感染产生抗体反应。有趣的是，约一半确诊为IA的患者入院时抗曲霉抗体滴度较高，这表明他们在入院时就已被感染。这些结果表明，重组RNU、CAT和DPPV在免疫功能低下和免疫功能正常患者的所有形式曲霉病的血清诊断中具有巨大潜力。
