将基于单链猿猴病毒40（SV40）的穿梭载体转化为双链形式，在猴细胞中并不需要SV40 T抗原。
Conversion of a single-stranded simian virus 40 (SV40)-based shuttle vector to its double-stranded form does not require the SV40 T antigen in monkey cells.
作者信息
Madzak C, Sarasin A
机构信息
Laboratory of Molecular Genetics, Institut de Recherches Scientifiques sur le Cancer, Villejuif, France.
出版信息
J Gen Virol. 1991 Dec;72 ( Pt 12):3091-3. doi: 10.1099/0022-1317-72-12-3091.
PMID:1662700
Abstract
We have transfected the single-stranded DNA form of the simian virus 40 (SV40)-based shuttle vector pZ189 into CV1P simian cells. Although the strand used did not code for the T antigen, we observed its conversion to a double-stranded DNA form. We deduced that the replication step converting a circular single-stranded DNA to a double-stranded one is independent of the SV40 T antigen in simian cells.
摘要
我们已将基于猿猴病毒40（SV40）的穿梭载体pZ189的单链DNA形式转染到CV1P猿猴细胞中。尽管所使用的链不编码T抗原，但我们观察到它转化为双链DNA形式。我们推断，在猿猴细胞中，将环状单链DNA转化为双链DNA的复制步骤与SV40 T抗原无关。
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