[整合到重组质粒中的病毒SV40调节区的功能活性]
[The functional activity of the regulator region of virus SV40 integrated into recombinant plasmids].
作者信息
Landau S M, Kolomiets L I, Tikhonov A V
出版信息
Tsitol Genet. 1994 Mar-Apr;28(2):55-62.
PMID:7941017
Abstract
We propose the system for transfection of eucaryotic SV40-based vectors. The method comprises simultaneous transfection of permissive monkey cells with plasmid DNA and the virus SV40. It is likely that the induction of replication of recombinant plasmids by the SV40 T-antigen is an indicator of functional activity of the SV40 regulatory region integrated into recombinant plasmids.
摘要
我们提出了用于真核生物基于SV40的载体转染的系统。该方法包括用质粒DNA和病毒SV40同时转染允许的猴细胞。SV40 T抗原诱导重组质粒复制很可能是整合到重组质粒中的SV40调控区功能活性的一个指标。
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