Bernini Andrea, Spiga Ottavia, Ciutti Arianna, Venditti Vincenzo, Prischi Filippo, Governatori Mariangela, Bracci Luisa, Lelli Barbara, Pileri Silvia, Botta Mauro, Barge Alessandro, Laschi Franco, Niccolai Neri
Biomolecular Structure Research Center and Dipartimento di Biologia Molecolare, Università di Siena, Via A. Fiorentina, I-53100 Siena, Italy.
Biochim Biophys Acta. 2006 May;1764(5):856-62. doi: 10.1016/j.bbapap.2006.02.013. Epub 2006 Apr 3.
Paramagnetic probes, whose approach to proteins can be monitored by nuclear magnetic resonance (NMR) studies, have been found of primary relevance for investigating protein surfaces accessibility. Here, paramagnetic probes are also suggested for a systematic investigation on protein aggregation. Bovine pancreatic trypsin inhibitor (BPTI) was used as a model system for aggregation by analyzing its interaction with TEMPOL and Gd(III)DTPA-BMA. Some of the measured paramagnetic relaxation rates of BPTI protons exhibited a reverse dependence on protein concentration, which can be attributed to the formation of transient BPTI aggregates.
顺磁探针可通过核磁共振（NMR）研究来监测其与蛋白质的结合方式，已发现其在研究蛋白质表面可及性方面具有重要意义。在此，还建议使用顺磁探针来系统研究蛋白质聚集。通过分析牛胰蛋白酶抑制剂（BPTI）与TEMPOL和钆（III）二乙烯三胺五乙酸-双甲基丙烯酸酯（Gd(III)DTPA-BMA）的相互作用，将其用作聚集的模型系统。BPTI质子的一些测量顺磁弛豫率表现出与蛋白质浓度的反向依赖性，这可归因于瞬时BPTI聚集体的形成。
