Levandowski R A, Horohov D W
Division of Virology, Center for Biologics Evaluation and Research, Bethesda, Maryland 20892.
J Med Virol. 1991 Oct;35(2):116-20. doi: 10.1002/jmv.1890350208.
Natural killer-like cellular cytotoxicity was augmented by incubation of human rhinovirus serotype 2 with peripheral blood mononuclear leukocytes collected from healthy donors. The production of alpha interferon but not gamma interferon was identified in the same cell cultures. A specific interaction of conformationally intact rhinovirus with peripheral blood mononuclear leukocytes was required for induction of the response, since the response was extinguished at reduced quantities of infectious rhinovirus, and acid inactivated rhinovirus did not augment cellular cytotoxicity. Productive replication of rhinovirus was not observed in cultures of peripheral blood mononuclear leukocytes. The replicative failure was not related merely to interferon production, since the rate of disappearance of rhinovirus was similar to that observed in cell free medium. The findings suggest that natural killer cells should be considered as a potential component of the local nasopharyngeal pathophysiology of rhinovirus infection.
通过将2型人鼻病毒与从健康供体采集的外周血单个核白细胞共同孵育，自然杀伤样细胞毒性增强。在相同的细胞培养物中鉴定出α干扰素而非γ干扰素的产生。诱导该反应需要构象完整的鼻病毒与外周血单个核白细胞的特异性相互作用，因为在感染性鼻病毒数量减少时反应消失，且酸灭活的鼻病毒不会增强细胞毒性。在外周血单个核白细胞培养物中未观察到鼻病毒的有效复制。复制失败不仅仅与干扰素产生有关，因为鼻病毒消失的速率与在无细胞培养基中观察到的相似。这些发现表明，自然杀伤细胞应被视为鼻病毒感染局部鼻咽病理生理学的潜在组成部分。
