Satoh K, Sawai T, Shimosegawa T, Koizumi M, Yamazaki T, Mochizuki F, Toyota T
Department of Pathology, Tohoku University Hospital.
Nihon Shokakibyo Gakkai Zasshi. 1991 Nov;88(11):2795-803.
The point mutation of c-K-ras oncogene at codon 12 was investigated in 15 cases of mucin-producing cystic tumor of the pancreas by a modified polymerase chain reaction (PCR) method. Three percent formalin-fixed and paraffin embedded materials were employed for the present investigation. These fifteen cases were histopathologically classified into five categories (from group I to V) according to Kozuka's classification which was based on cell atypia of the tumor cells; 5 cases were judged as group V, 4 cases as group IV, and 6 cases as group III. The frequency of point mutation was 5/5 in group V, 3/4 in group IV, and 2/6 in group III. The frequency of point mutation was correlated with morphologically determined cell atypia. Among group III, the point mutation was noticed in the cases with larger tumor size. From genetical point of view, the results indicated more malignant biological feature of mucin-producing cystic tumor of the pancreas than has generally been accepted.
采用改良聚合酶链反应（PCR）方法，对15例胰腺黏液性囊性肿瘤患者进行c-K-ras癌基因第12密码子点突变检测。本研究采用3%中性福尔马林固定、石蜡包埋组织。根据Kozuka基于肿瘤细胞异型性的分类方法，这15例患者的组织病理学分类为5类（从I组到V组）；V组5例，IV组4例，III组6例。V组点突变率为5/5，IV组为3/4，III组为2/6。点突变率与形态学确定的细胞异型性相关。在III组中，肿瘤体积较大的病例出现点突变。从遗传学角度来看，结果表明胰腺黏液性囊性肿瘤的生物学特性比普遍认为的更具恶性。
