来自蓝藻节旋藻属PCC 7601的两个插入序列IS701和IS702的特征分析。

Characterization of two insertion sequences, IS701 and IS702, from the cyanobacterium Calothrix species PCC 7601.

作者信息

Mazel D, Bernard C, Schwarz R, Castets A M, Houmard J, Tandeau de Marsac N

机构信息

Unité de Physiologie Microbienne (CNRS, URA1129), Institut Pasteur, Paris, France.

出版信息

Mol Microbiol. 1991 Sep;5(9):2165-70. doi: 10.1111/j.1365-2958.1991.tb02146.x.

DOI:10.1111/j.1365-2958.1991.tb02146.x

PMID:1662761

Abstract

We describe the characterization of two insertion elements, IS701 and IS702, isolated from Calothrix species PCC 7601. These insertion elements were cloned from spontaneous pigmentation mutants. Both show the characteristics of typical bacterial insertion sequences, i.e. they present long terminal inverted repeats and they duplicate target DNA upon insertion. These elements share no homology with the only other cyanobacterial insertion sequence described so far, IS891. At least 15 copies of IS701 and 9 copies of IS702 were detected by hybridization experiments in the Calothrix 7601 genome. Their occurrence in several cyanobacterial strains is also reported.

摘要

我们描述了从眉藻属物种PCC 7601中分离出的两个插入元件IS701和IS702的特征。这些插入元件是从自发色素沉着突变体中克隆出来的。两者都表现出典型细菌插入序列的特征，即它们具有长末端反向重复序列，并且在插入时会复制靶DNA。这些元件与迄今为止描述的唯一其他蓝藻插入序列IS891没有同源性。通过杂交实验在眉藻7601基因组中检测到至少15个IS701拷贝和9个IS702拷贝。还报道了它们在几种蓝藻菌株中的存在情况。