Mengaud J, Dramsi S, Gouin E, Vazquez-Boland J A, Milon G, Cossart P
Unité de Génie Microbiologique, Institut Pasteur, Paris, France.
Mol Microbiol. 1991 Sep;5(9):2273-83. doi: 10.1111/j.1365-2958.1991.tb02158.x.
Evidence for pleiotropic activation of virulence genes in Listeria monocytogenes is presented. A complementation study of a spontaneous prfA-deletion mutant and analysis of cassette and transposon insertion mutants showed that the gene prfA activates the transcription of four independent genes which code for a phosphatidyl-inositol-specific phospholipase C (gene plcA), listeriolysin O (gene hlyA), a metallo-protease (gene prtA) and a lecithinase (gene prtC). Transcription of prfA is not constitutive. During the growth phase, two peaks of prfA transcript accumulation were observed: the first was during exponential growth, and the second was at the beginning of the stationary phase. In addition, two prfA-specific transcripts of 2.2 kb and 1 kb are detected. Early in exponential growth, prfA is co-transcribed with plcA which lies upstream prfA, giving rise to the 2.2 kb plcA-prfA transcript. In late-exponential growth and at the beginning of the stationary phase, prfA transcripts of 1 kb are predominantly detected. Our results demonstrate that since prfA controls plcA transcription, it also regulates its own synthesis.
本文提供了单核细胞增生李斯特菌毒力基因多效性激活的证据。对自发缺失prfA的突变体进行互补研究，并对盒式和转座子插入突变体进行分析，结果表明prfA基因激活了四个独立基因的转录，这四个基因分别编码磷脂酰肌醇特异性磷脂酶C（plcA基因）、李斯特菌溶血素O（hlyA基因）、金属蛋白酶（prtA基因）和卵磷脂酶（prtC基因）。prfA的转录不是组成型的。在生长阶段，观察到prfA转录本积累出现两个峰值：第一个峰值出现在指数生长期，第二个峰值出现在稳定期开始时。此外，还检测到两种大小分别为2.2 kb和1 kb的prfA特异性转录本。在指数生长早期，prfA与位于其上游的plcA共转录，产生2.2 kb的plcA-prfA转录本。在指数生长后期和稳定期开始时，主要检测到1 kb的prfA转录本。我们的结果表明，由于prfA控制plcA的转录，它也调节自身的合成。
