Cavaliere V, Graziani F, Andone S, Manzi A, Malva C
Istituto Internazionale di Genetica e Biofisca, CNR, Naples, Italy.
Mol Gen Genet. 1991 Dec;230(3):433-41. doi: 10.1007/BF00280300.
The abnormal oocyte phenotype is characterized by instability, as shown by the loss and reappearance of the abo maternal effect under specific genetic conditions. Our previous finding that a correlation exists between the abo phenotype and the presence of a blood transposon in region 32E, led us to perform an extensive genetic and molecular analysis of the most significant aspects of the abo phenotype in different genetic backgrounds. The results of these experiments can be summarized as follows: Complete reversion occurs only when the blood trnasposon is lost, thus definitively demonstrating that the insertion of the blood transposon in region 32E is the molecular event that causes the pleiotropic abo phenotype. Partial reversion can also occur without loss of the transposon indicating that different molecular pathways may be involved in the loss of the abo phenotype. Reappearance of the full abo phenotype can occur only in heterozygous lines constructed from partially revertant abo homozygous lines that have not lost the blood transposon.
异常卵母细胞表型的特征是不稳定，这在特定遗传条件下abo母体效应的丧失和重现中得到体现。我们之前发现abo表型与32E区域中一个血液转座子的存在之间存在相关性，这促使我们在不同遗传背景下对abo表型的最重要方面进行广泛的遗传和分子分析。这些实验的结果可以总结如下：只有当血液转座子丢失时才会发生完全回复，从而明确证明32E区域中血液转座子的插入是导致多效性abo表型的分子事件。在转座子未丢失的情况下也可能发生部分回复，这表明不同的分子途径可能参与了abo表型的丧失。完全abo表型的重现仅发生在由未丢失血液转座子的部分回复abo纯合系构建的杂合系中。
