Graziani F, Vicari L, Boncinelli E, Malva C, Manzi A, Mariani C
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7662-4. doi: 10.1073/pnas.78.12.7662.
Drosophila melanogaster females homozygous for the abnormal oocyte mutation produce a large excess of female offspring when crossed with XY/0 males. After several generations in abo homozygous stock, this maternal effect is no longer observed. The disappearance of the abo phenotype is coupled with an increase in the amount of DNA coding for rRNA (rDNA). We have used restriction endonuclease analysis of total DNA extracted from adult females and from single female larval brains to investigate the molecular organization of rDNA before and after the loss of abo phenotype. The rDNA increase is associated with variations of the restriction pattern of the nontranscribed spacer, probably due to a selective increase of rDNA repeats.
异常卵母细胞突变纯合的黑腹果蝇雌性与XY/0雄性杂交时会产生大量多余的雌性后代。在abo纯合品系中经过几代后，这种母体效应不再被观察到。abo表型的消失与编码rRNA（rDNA）的DNA量增加有关。我们使用从成年雌性和单个雌性幼虫大脑中提取的总DNA的限制性内切酶分析，来研究abo表型丧失前后rDNA的分子组织。rDNA的增加与非转录间隔区限制性图谱的变化有关，这可能是由于rDNA重复序列的选择性增加所致。
