Yoon Jung-Hoon, Kang So-Jung, Lee Mi-Hwa, Oh Hyun Woo, Oh Tae-Kwang
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
Int J Syst Evol Microbiol. 2006 May;56(Pt 5):1079-1083. doi: 10.1099/ijs.0.63840-0.
A Gram-negative, non-motile, non-spore-forming, slightly halophilic bacterial strain, DSW-74T, was isolated from sea water off the island of Dokdo, Korea, and its taxonomic position was investigated by a polyphasic study. Strain DSW-74T grew optimally at 37 degrees C and in the presence of 2 % (w/v) NaCl. It contained Q-10 as the predominant ubiquinone and C17 : 1omega6c and C18 : 1omega7c as the major fatty acids. Its DNA G+C content was 65.8 mol%. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain DSW-74T was most closely related to Porphyrobacter species. Similarity values between the 16S rRNA gene sequence of strain DSW-74T and those of the type strains of recognized Porphyrobacter species and of Erythromicrobium ramosum were in the range 97.4-98.7 %. Strain DSW-74T exhibited 16S rRNA gene sequence similarity values of <97.5 % to recognized Erythrobacter species and the other species used in the phylogenetic analysis. DNA-DNA relatedness levels and differential phenotypic properties made it possible to categorize strain DSW-74T as representing a novel Porphyrobacter species. On the basis of the taxonomic data presented, it is proposed that DSW-74T (=KCTC 12395T=DSM 17193T) should be classified in the genus Porphyrobacter as the type strain of a novel species, Porphyrobacter dokdonensis sp. nov.
从韩国独岛附近海水分离出一株革兰氏阴性、无运动性、不形成芽孢、轻度嗜盐的细菌菌株DSW-74T，并通过多相研究对其分类地位进行了调查。菌株DSW-74T在37℃和2%（w/v）NaCl存在的条件下生长最佳。它以Q-10作为主要泛醌，以C17 : 1ω6c和C18 : 1ω7c作为主要脂肪酸。其DNA G+C含量为65.8 mol%。基于16S rRNA基因序列的系统发育分析表明，菌株DSW-74T与卟啉杆菌属物种关系最为密切。菌株DSW-74T的16S rRNA基因序列与已确认的卟啉杆菌属物种模式菌株以及分枝赤微菌的16S rRNA基因序列相似性值在97.4-98.7%范围内。菌株DSW-74T与已确认的红杆菌属物种以及系统发育分析中使用的其他物种的16S rRNA基因序列相似性值<97.5%。DNA-DNA相关性水平和不同的表型特征使得可以将菌株DSW-74T归类为代表一个新的卟啉杆菌属物种。根据所提供的分类学数据，建议将DSW-74T（=KCTC 12395T=DSM 17193T）分类到卟啉杆菌属，作为一个新物种——独岛卟啉杆菌（Porphyrobacter dokdonensis sp. nov.）的模式菌株。
