Yoon Jung-Hoon, Kang So-Jung, Lee Soo-Young, Lee Choong-Hwan, Oh Tae-Kwang
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
Int J Syst Evol Microbiol. 2005 Sep;55(Pt 5):2051-2055. doi: 10.1099/ijs.0.63777-0.
Two Gram-negative, non-spore-forming, slightly halophilic gliding bacterial strains, DSW-8(T) and DSW-9, were isolated from sea water off a Korean island, Dokdo, of the East Sea, Korea, and their taxonomic position was investigated by a polyphasic study. The two strains grew optimally at 30 degrees C and in the presence of 2-3% (w/v) NaCl. Strains DSW-8(T) and DSW-9 were characterized chemotaxonomically as containing MK-6 as the predominant menaquinone and iso-C(17:0) 3-OH, iso-C(15:0) and iso-C(15:1) as the major fatty acids. Major polar lipids were phosphatidylethanolamine, two unidentified phospholipids, an unidentified glycolipid and an amino group-containing lipid that was ninhydrin-positive. Their DNA G+C contents were 36.1 and 35.9 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains DSW-8(T) and DSW-9 fell within the genus Maribacter of the family Flavobacteriaceae. Strains DSW-8(T) and DSW-9 exhibited no difference in their 16S rRNA gene sequences and possessed a mean DNA-DNA relatedness level of 89%. Strains DSW-8(T) and DSW-9 exhibited 16S rRNA gene sequence similarity levels of 96.9-98.0% to the type strains of the four recognized Maribacter species, but their low level of DNA-DNA relatedness with these species demonstrated that they constitute a distinct Maribacter species. On the basis of phenotypic and phylogenetic data and genetic distinctiveness, strains DSW-8(T) (=KCTC 12393(T)=DSM 17201(T)) and DSW-9 were classified in the genus Maribacter as members of a novel species, for which the name Maribacter dokdonensis sp. nov. is proposed.
从韩国东海独岛附近海域分离出两株革兰氏阴性、不形成芽孢、轻度嗜盐的滑行细菌菌株，DSW-8(T) 和 DSW-9，并通过多相研究对其分类地位进行了调查。这两株菌株在30℃以及2-3%（w/v）NaCl 存在的条件下生长最佳。菌株 DSW-8(T) 和 DSW-9 的化学分类特征为：主要的甲基萘醌为 MK-6，主要脂肪酸为 iso-C(17:0) 3-OH、iso-C(15:0) 和 iso-C(15:1)。主要极性脂为磷脂酰乙醇胺、两种未鉴定的磷脂、一种未鉴定的糖脂以及一种茚三酮阳性的含氨基脂。它们的 DNA G+C 含量分别为 36.1 和 35.9 mol%。基于 16S rRNA 基因序列的系统发育分析表明，菌株 DSW-8(T) 和 DSW-9 属于黄杆菌科海杆菌属。菌株 DSW-8(T) 和 DSW-9 的 16S rRNA 基因序列没有差异，平均 DNA-DNA 相关性水平为 89%。菌株 DSW-8(T) 和 DSW-9 与四个已确认的海杆菌属物种的模式菌株的 16S rRNA 基因序列相似性水平为 96.9-98.0%，但它们与这些物种的低水平 DNA-DNA 相关性表明它们构成一个独特的海杆菌属物种。基于表型、系统发育数据和遗传独特性，菌株 DSW-8(T)（=KCTC 12393(T)=DSM 17201(T)）和 DSW-9 被归入海杆菌属，作为一个新物种的成员，为此提出新种名为独岛海杆菌（Maribacter dokdonensis sp. nov.）。
