Paredez Alexander R, Somerville Christopher R, Ehrhardt David W
Department of Biological Sciences, Stanford University, 260 Panama Street, Stanford, CA 94305, USA.
Science. 2006 Jun 9;312(5779):1491-5. doi: 10.1126/science.1126551. Epub 2006 Apr 20.
Expression of a functional yellow fluorescent protein fusion to cellulose synthase (CESA) in transgenic Arabidopsis plants allowed the process of cellulose deposition to be visualized in living cells. Spinning disk confocal microscopy revealed that CESA complexes in the plasma membrane moved at constant rates in linear tracks that were aligned and were coincident with cortical microtubules. Within each observed linear track, complex movement was bidirectional. Inhibition of microtubule polymerization changed the fine-scale distribution and pattern of moving CESA complexes in the membrane, indicating a relatively direct mechanism for guidance of cellulose deposition by the cytoskeleton.
在转基因拟南芥植物中，功能性黄色荧光蛋白与纤维素合酶（CESA）融合表达，使得纤维素沉积过程能够在活细胞中可视化。旋转盘共聚焦显微镜显示，质膜中的CESA复合体在线性轨道上以恒定速率移动，这些轨道排列整齐且与皮层微管重合。在每个观察到的线性轨道内，复合体的移动是双向的。微管聚合的抑制改变了膜中移动的CESA复合体的精细分布和模式，表明细胞骨架对纤维素沉积的引导存在相对直接的机制。
