Petrella A, Festa M, Ercolino S F, Zerilli M, Stassi G, Solito E, Parente L
Department of Pharmaceutical Sciences, University of Salerno, Salerno, Italy.
Cancer Biol Ther. 2006 Jun;5(6):643-7. doi: 10.4161/cbt.5.6.2700. Epub 2006 Jun 11.
We investigated the expression of annexin-1 (ANXA1) in thyroid carcinoma cell lines and in thyroid cancers with a different degree of differentiation. The highest level of ANXA1 expression examined by Western blotting was detected in the papillary carcinoma cells (NPA) and in the follicular cells (WRO). On the other hand, the most undifferentiated thyroid carcinoma cells (ARO and FRO) presented the lowest level of ANXA1 expression. In surgical tissue specimens from 32 patients with thyroid cancers, we found high immunoreactivity for ANXA1 in papillary (PTC) and follicular (FTC) thyroid cancers while in undifferentiated thyroid cancers (UTC) the expression of the protein was barely detectable. Control thyroid tissue resulted positive for ANXA1. In summary, 70% of UTC examined weakly expressed ANXA1, whereas 65% of PTC or FTC specimens tested showed high expression of the protein. Thus ANXA1 expression may correlate with the tumorigenesis suggesting that the protein may represent an effective differentiation marker in thyroid cancer.
我们研究了膜联蛋白-1（ANXA1）在甲状腺癌细胞系以及不同分化程度的甲状腺癌中的表达情况。通过蛋白质印迹法检测到，在乳头状癌细胞（NPA）和滤泡细胞（WRO）中，ANXA1的表达水平最高。另一方面，分化程度最低的甲状腺癌细胞（ARO和FRO）中ANXA1的表达水平最低。在32例甲状腺癌患者的手术组织标本中，我们发现乳头状甲状腺癌（PTC）和滤泡状甲状腺癌（FTC）中ANXA1具有高免疫反应性，而在未分化甲状腺癌（UTC）中几乎检测不到该蛋白的表达。对照甲状腺组织ANXA1呈阳性。总之，70%的检测UTC中ANXA1表达较弱，而65%的检测PTC或FTC标本中该蛋白表达较高。因此，ANXA1的表达可能与肿瘤发生相关，提示该蛋白可能是甲状腺癌中一种有效的分化标志物。
