Department of General Surgery, Fuyang Hospital Affiliated to Anhui Medical University, Fuyang, Anhui, China.
Department of Burn and Plastic Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.
Bioengineered. 2021 Dec;12(2):9251-9265. doi: 10.1080/21655979.2021.1996511.
Thyroid carcinoma is one of the most common endocrine malignancies, in which papillary thyroid carcinoma (PTC) is the main pathotype. ANXA1 plays a significant role in many cancer types, but how it works in PTC has not been identified. MYC is a common transcript factor involved in tumorigenesis, development, invasion, and metastasis. The relation between ANXA1 and MYC has not been proved in PTC. In this study, firstly, we analyzed the expression and prognostic value of ANXA1 in pan-cancer using the data from the UCSC database. Then we explore the role of ANXA1 in PTC, including expression, prognostic value, and immune infiltration. In addition, we evaluated the relation between ANXA1 and the transcription factor MYC. Finally, we identified the expression of ANXA1 and MYC and then evaluated their function associated with proliferation and apoptosis in PTC cell lines by CCK8 proliferation and flow cytometry apoptosis experiment. We found that ANXA1 is up-regulated in PTC comparing with normal patients. High expression of ANXA1 was associated with adverse overall survival of PTC. ANXA1 may be regulated by MYC to promote the proliferation of PTC. MYC may regulate the expression of ANXA and thus affect the proliferation of PTC.
甲状腺癌是最常见的内分泌恶性肿瘤之一，其中甲状腺乳头状癌（PTC）是主要的病理类型。ANXA1 在许多癌症类型中都起着重要作用，但它在 PTC 中的作用尚未确定。MYC 是一种常见的转录因子，参与肿瘤发生、发展、侵袭和转移。在 PTC 中，ANXA1 与 MYC 之间的关系尚未得到证实。在这项研究中，首先，我们使用 UCSC 数据库中的数据分析了 ANXA1 在泛癌中的表达和预后价值。然后，我们探讨了 ANXA1 在 PTC 中的作用，包括表达、预后价值和免疫浸润。此外，我们评估了 ANXA1 与转录因子 MYC 之间的关系。最后，我们通过 CCK8 增殖实验和流式细胞术凋亡实验鉴定了 PTC 细胞系中 ANXA1 和 MYC 的表达，并评估了它们与增殖和凋亡相关的功能。我们发现，与正常患者相比，PTC 中 ANXA1 的表达上调。ANXA1 的高表达与 PTC 的不良总生存率相关。ANXA1 可能受 MYC 调控，促进 PTC 的增殖。MYC 可能调节 ANXA 的表达，从而影响 PTC 的增殖。
